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Journal of Clinical Microbiology, April 2006, p. 1578-1580, Vol. 44, No. 4
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.4.1578-1580.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

A 24-Hour Screening Protocol for Identification of Vancomycin-Resistant Enterococcus faecium

Steven J. Drews,1,2 Grant Johnson,1 Farhad Gharabaghi,1 Margaret Roscoe,1 Anne Matlow,2,3 Raymond Tellier,1,2 and Susan E. Richardson1,2*

Division of Microbiology, Hospital for Sick Children, Toronto, ON, Canada,1 Department of Pathobiology and Laboratory Medicine, University of Toronto, Toronto, ON, Canada,2 Department of Paediatrics, Hospital for Sick Children, Toronto, ON, Canada3

Received 12 October 2005/ Returned for modification 29 October 2005/ Accepted 14 January 2006

We describe a 24-h protocol for the identification of patients who are positive for vancomycin-resistant Enterococcus faecium (VRE), using stool and rectal swab samples and VRE screening broth, automated DNA extraction, and real-time PCR for vanA and vanB genes. Compared to conventional screening methods, this protocol had a high sensitivity and specificity and a negative predictive value.

* Corresponding author. Mailing address: Department of Paediatric Laboratory Medicine, The Hospital for Sick Children, 555 University Ave., Room 3654, Toronto, Ontario M5G 1X8, Canada. Phone 416-813-5992. Fax: 416-813-6257. E-mail: susan.richardson{at}sickkids.ca.

Journal of Clinical Microbiology, April 2006, p. 1578-1580, Vol. 44, No. 4
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.4.1578-1580.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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