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Journal of Clinical Microbiology, May 2006, p. 1733-1739, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1733-1739.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Comparison between the Hybrid Capture II Test and an SPF1/GP6+ PCR-Based Assay for Detection of Human Papillomavirus DNA in Cervical Swab Samples

Shang-Lang Huang,¹ Angel Chao,^1,2 Swei Hsueh,³ Fang-Yu Chao,¹ Chu-Chun Huang,¹ Jung-Erh Yang,¹ Ching-Yu Lin,⁴ Chiu-Cho Yan,⁴ Hung-Hsueh Chou,¹ Kuan-Gen Huang,¹ Huei-Jean Huang,¹ Tzu-I Wu,¹ Mao-Jung Tseng,¹ Jian-Tai Qiu,¹ Cheng-Tao Lin,¹ Ting-Chang Chang,¹ and Chyong-Huey Lai^1*

Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Taoyuan, Taiwan,¹ Graduate Institute of Clinical Medical Sciences, Chang Gung University, Taoyuan, Taiwan,² Department of Pathology, Chang Gung Memorial Hospital, Taoyuan, Taiwan,³ Yuan-Shan Research Institute, King Car Food Inc., I-Lan, Taiwan⁴

Received 7 October 2005/ Returned for modification 10 February 2006/ Accepted 1 March 2006

We compared the efficacy of human papillomavirus (HPV) DNA detection between a PCR-based genechip (Easychip HPV Blot [hereafter referred to as HPV Blot]; King Car, Taiwan) method and Hybrid Capture II (HCII; Digene, Gaithersburg, MD) in women with previous normal (n = 146) or abnormal (atypical squamous cells of undetermined significance [ASCUS] [n = 208]) cytology. A total of 354 cervical swab samples were collected for HPV DNA assay by both HCII and SPF1/GP6+ PCR followed by HPV Blot tests. Colposcopy-directed biopsy was performed if clinically indicated. Of the 354 samples, HPV-positive rates by these two methods (HCII and HPV Blot) were 12.6% and 18.2% in 143 normal samples, 36.2% and 45.7% in 105 ASCUS samples, 57.4% and 57.4% in 94 low-grade squamous intraepithelial lesion samples, and 83.3% and 75.0% in 12 high-grade squamous intraepithelial lesion samples, respectively. The concordance of HPV Blot and HCII was 80.8% (286/354), and the agreement between the methods ( value, 0.68) was substantial. Discrepancies were further investigated by at least one of the following three methods: direct sequencing, type-specific PCR, and HPV Blot genotyping of cervical biopsy tissue. In the 15 HCII-positive samples, HPV Blot detected only non-HCII HPV genotypes; results of further verification methods were consistent with the latter test in the 15 samples. Of the 20 samples with HCII-negative and HPV Blot-positive results, 18 were found to contain the 13 HCII high-risk genotypes by verification methods. In only 16.7% (3/18) of the HCII-positive but HPV Blot-negative samples, further studies detected the 13 HCII genotypes. We conclude that HPV Blot seemed comparable to HCII for detection of HPV DNA in cervical swab samples.

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* Corresponding author. Mailing address: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, 5 Fu-Shin St., Kueishan, Taoyuan 333, Taiwan. Phone: 886-3-328-1200. Fax: 886-3-328-8252. E-mail: sh46erry{at}ms6.hinet.net.

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Journal of Clinical Microbiology, May 2006, p. 1733-1739, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1733-1739.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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