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Journal of Clinical Microbiology, May 2006, p. 1828-1830, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1828-1830.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Rapid Detection of Brucella spp. in Blood Cultures by Fluorescence In Situ Hybridization

Nele Wellinghausen,1 Karsten Nöckler,2 Anja Sigge,1 Melanie Bartel,1 Andreas Essig,1 and Sven Poppert1*

Department of Medical Microbiology and Hygiene, University Hospital of Ulm, Ulm, Germany,1 Federal Institute for Risk Assessment, Berlin, Germany2

Received 9 August 2005/ Returned for modification 23 December 2005/ Accepted 10 March 2006

Brucellosis is a severe systemic disease in humans. We describe a new 16S rRNA-based fluorescence in situ hybridization assay that facilitates rapid and specific detection of all human pathogenic species of Brucella and that can be applied directly to positive blood cultures.

* Corresponding author. Mailing address: Department of Medical Microbiology and Hygiene, University Hospital of Ulm, Robert-Koch-Str. 8, 89081 Ulm, Germany. Phone: 49-731-50024610. Fax: 49-731-50023473. E-mail: sven.poppert{at}medizin.uni-ulm.de.

Journal of Clinical Microbiology, May 2006, p. 1828-1830, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1828-1830.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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