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Journal of Clinical Microbiology, May 2006, p. 1875-1876, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1875-1876.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Presence of a Novel DNA Methylation Enzyme in Methicillin-Resistant Staphylococcus aureus Isolates Associated with Pig Farming Leads to Uninterpretable Results in Standard Pulsed-Field Gel Electrophoresis Analysis

Corina C. P. M. Bens, Andreas Voss, and Corné H. W. Klaassen*

Department of Medical Microbiology and Infectious Diseases, Regional Public Health Laboratory, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands

Received 13 January 2006/ Accepted 23 February 2006

Genomic DNA from methicillin-resistant Staphylococcus aureus isolates recovered from pigs and their caretakers proved resistant to SmaI digestion, leading to uninterpretable results in standard pulsed-field gel electrophoresis. This is the result of a yet unknown restriction/methylation system in the genus Staphylococcus with the recognition sequence CCNGG.

* Corresponding author. Mailing address: Department of Medical Microbiology and Infectious Diseases, Regional Public Health Laboratory, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands. Phone: 31 24 3657514. Fax: 31 24 3657516. E-mail: c.klaassen{at}cwz.nl.

Journal of Clinical Microbiology, May 2006, p. 1875-1876, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1875-1876.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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