This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kong, F.
Right arrow Articles by Gilbert, G. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kong, F.
Right arrow Articles by Gilbert, G. L.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 2006, p. 1887-1891, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1887-1891.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Multiplex PCR-Based Reverse Line Blot Hybridization Assay To Identify 23 Streptococcus pneumoniae Polysaccharide Vaccine Serotypes

Fanrong Kong,1 Mitchell Brown,1,2 Archcna Sabananthan,1,3 Xianyu Zeng,1,4 and Gwendolyn L. Gilbert1*

Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead, New South Wales, Australia,1 Department of Cell and Molecular Biology, Faculty of Science, University of Technology, Sydney, New South Wales, Australia,2 School of Biotechnology and Biomolecular Sciences, The University of New South Wales, New South Wales, Australia,3 Wuhan First Hospital, Hubei Province, Wuhan 430022, People's Republic of China4

Received 11 November 2005/ Returned for modification 28 January 2006/ Accepted 27 February 2006

We developed a multiplex PCR-based reverse line blot assay to identify 23 pneumococcal serotypes represented in the polysaccharide vaccine, using 334 well-characterized isolates, representing all 90 serotypes, and 268 "unknowns." The assay identified all target serotypes, but 11, which cross-react with 1 to 4 nonvaccine serotypes, could be distinguished using serotype-specific antisera.

* Corresponding author. Mailing address: Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research (ICPMR), Westmead Hospital, Darcy Road, Westmead, New South Wales 2145, Australia. Phone: (612) 9845-6255. Fax: (612) 9893-8659. E-mail: lyng{at}icpmr.wsahs.nsw.gov.au.

Journal of Clinical Microbiology, May 2006, p. 1887-1891, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1887-1891.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zhou, F., Kong, F., McPhie, K., Ratnamohan, M., Donovan, L., Zeng, F., Gilbert, G. L., Dwyer, D. E. (2009). Identification of 20 Common Human Enterovirus Serotypes by Use of a Reverse Transcription-PCR-Based Reverse Line Blot Hybridization Assay. J. Clin. Microbiol. 47: 2737-2743 [Abstract] [Full Text]  
  • Jin, P., Xiao, M., Kong, F., Oftadeh, S., Zhou, F., Liu, C., Gilbert, G. L. (2009). Simple, Accurate, Serotype-Specific PCR Assay To Differentiate Streptococcus pneumoniae Serotypes 6A, 6B, and 6C. J. Clin. Microbiol. 47: 2470-2474 [Abstract] [Full Text]  
  • Cai, L., Kong, F., Wang, Q., Wang, H., Xiao, M., Sintchenko, V., Gilbert, G. L. (2009). A new multiplex PCR-based reverse line-blot hybridization (mPCR/RLB) assay for rapid staphylococcal cassette chromosome mec (SCCmec) typing. J Med Microbiol 58: 1045-1057 [Abstract] [Full Text]  
  • Rivera-Olivero, I. A., Blommaart, M., Bogaert, D., Hermans, P. W. M., de Waard, J. H. (2009). Multiplex PCR reveals a high rate of nasopharyngeal pneumococcal 7-valent conjugate vaccine serotypes co-colonizing indigenous Warao children in Venezuela. J Med Microbiol 58: 584-587 [Abstract] [Full Text]  
  • Azzari, C., Moriondo, M., Indolfi, G., Massai, C., Becciolini, L., de Martino, M., Resti, M. (2008). Molecular detection methods and serotyping performed directly on clinical samples improve diagnostic sensitivity and reveal increased incidence of invasive disease by Streptococcus pneumoniae in Italian children. J Med Microbiol 57: 1205-1212 [Abstract] [Full Text]  
  • Zhou, F., Kong, F., Tong, Z., Gilbert, G. L. (2007). Identification of Less-Common Streptococcus pneumoniae Serotypes by a Multiplex PCR-Based Reverse Line Blot Hybridization Assay. J. Clin. Microbiol. 45: 3411-3415 [Abstract] [Full Text]  
  • Slotved, H.-C., Kong, F., Lambertsen, L., Sauer, S., Gilbert, G. L. (2007). Serotype IX, a Proposed New Streptococcus agalactiae Serotype. J. Clin. Microbiol. 45: 2929-2936 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»