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Journal of Clinical Microbiology, July 2006, p. 2338-2342, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00425-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Evaluation of the Genotype MTBDR Assay for Rapid Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis Isolates

Cengiz Cavusoglu,* Ajda Turhan, Pinar Akinci, and Ilknur Soyler

Ege University Medical Faculty, Department of Clinical Microbiology, Mycobacteriology Laboratory, 35100 Izmir, Turkey

Received 27 February 2006/ Returned for modification 4 April 2006/ Accepted 24 April 2006

A novel PCR-based reverse hybridization method Genotype MTBDR assay (Hain Lifescience GmbH, Nehren, Germany) was evaluated for rapid detection of rifampin (RIF) and isoniazid (INH) resistance in Turkish Mycobacterium tuberculosis isolates. The Genotype MTBDR assay is designed to detect mutations within the 81-bp hotspot region of rpoB and mutations at katG codon 315. A total of 41 RIF-resistant M. tuberculosis isolates with rpoB mutations that were previously tested by the INNO-LiPA Rif.TB kit and also characterized by DNA sequencing were included in the study. Thirty-seven of these isolates were also resistant to INH. RIF resistance was correctly identified in 39 of 41 isolates (95.1%) with the Genotype MTBDR assay probes specific for these mutations. One isolate with a Gln-490-His mutation and another one with a CGG insertion between codons 514 and 515 were identified as RIF sensitive by the Genotype MTBDR assay. While the INNO-LiPA Rif.TB kit was able to determine the CGG insertion between codons 514 and 515, the Gln-490-His mutation outside the 81-bp hotspot region was not detected by the INNO-LiPA Rif.TB kit. These isolates had MICs of ≥32 µg/ml for RIF. The Genotype MTBDR assay also correctly identified 27 of 37 INH-resistant isolates (73%) with mutations in katG codon 315. In conclusion, the Genotype MTBDR assay may be useful for the rapid diagnosis of the most common mutations found in multidrug-resistant M. tuberculosis strains. However, the test results should always be confirmed with phenotypic methods.

* Corresponding author. Mailing address: Ege University Medical Faculty, Department of Clinical Microbiology, Mycobacteriology Laboratory, 35100 Izmir, Turkey. Phone: 90 232 3903303. Fax: 90 232 3422142. E-mail: cengizc2003{at}yahoo.com.

Journal of Clinical Microbiology, July 2006, p. 2338-2342, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00425-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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