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Journal of Clinical Microbiology, July 2006, p. 2359-2366, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00447-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Spread of Escherichia coli Strains with High-Level Cefotaxime and Ceftazidime Resistance between the Community, Long-Term Care Facilities, and Hospital Institutions

Jesús Oteo,1 Carmen Navarro,1 Emilia Cercenado,2 Alberto Delgado-Iribarren,3 Isabel Wilhelmi,4 Beatriz Orden,5 Carmen García,1 Silvia Miguelañez,1 María Pérez-Vázquez,1 Silvia García-Cobos,1 Belén Aracil,1 Verónica Bautista,1 and José Campos1,6*

Antibiotic Laboratory, Bacteriology Service, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain,1 Microbiology Department, Hospital Gregorio Marañón, Madrid, Spain,2 Microbiology Department, Fundación Hospital de Alcorcón, Alcorcón, Madrid, Spain,3 Microbiology Department, Hospital Severo Ochoa, Leganés, Madrid, Spain,4 Microbiology Department, Centro de Especialidades Argüelles, Hospital Puerta de Hierro, Madrid, Spain,5 Consejo Superior de Investigaciones Científicas, Madrid, Spain6

Received 1 March 2006/ Returned for modification 27 April 2006/ Accepted 3 May 2006

A total of 151 Escherichia coli strains resistant to cefotaxime and ceftazidime were isolated during a prospective surveillance study. These strains were characterized by clinical, microbiological, and molecular analyses and were distributed into four clusters of 103, 11, 6, and 5 isolates, along with 25 unrelated strains. The principal cluster was isolated from urine, wound, blood, and other samples in three hospitals, eight nursing homes, and a community healthcare center. This cluster was associated with both nosocomial (65%) and community-acquired (35%) infections. Most strains were resistant to ciprofloxacin, gentamicin, tobramycin, cefepime, amoxicillin-clavulanic acid, and trimethoprim-sulfamethoxazole but were susceptible to imipenem. All isolates from the four clusters expressed the extended-spectrum ß-lactamase (ESBL) CTX-M-15. This enzyme was also present in 8 (30.8%) of the 26 unrelated isolates. The other ESBLs, CTX-M-14 and CTX-M-32, were detected in five and seven cases, respectively, but they were detected in individual E. coli isolates only. In three clusters, blaCTX-M-15 alleles were linked to an ISEcp1-like element, while in eight strains of cluster II an IS26 element preceded the blaCTX-M-15 allele. An additional pool of resistance genes included tetA, drfA14 or dfrA17, sul1 or sul2, aac(6')Ib, and aac(3)IIb. All except one of the 27 isolates tested for genetic virulence markers harbored the same three virulence genes: iutA and fyuA (siderophores), and traT (serum survival factor). Epidemic or occasional isolates of cefotaxime- and ceftazidime-resistant E. coli can spread between distinct health facilities including hospitals, community health centers, and long-term care centers.

* Corresponding author. Mailing address: Centro Nacional de Microbiología, Instituto de Salud Carlos III, Carretera Pozuelo a Majadahonda, 28220 Majadahonda, Madrid, Spain. Phone: (34) 91-509-7901. Fax: (34) 91-509-7966. E-mail: jcampos{at}isciii.es.

Journal of Clinical Microbiology, July 2006, p. 2359-2366, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00447-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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