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Journal of Clinical Microbiology, July 2006, p. 2575-2576, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00399-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Use of BBL CHROMagar MRSA Medium for Identification of Methicillin-Resistant Staphylococcus aureus Directly from Blood Cultures

John Pape,1 Jill Wadlin,1 and Irving Nachamkin1,2*

Clinical Microbiology Laboratory, Hospital of the University of Pennsylvania,1 Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-42832

Received 22 February 2006/ Returned for modification 10 April 2006/ Accepted 25 April 2006

We evaluated the ability of BBL CHROMagar MRSA medium (Becton Dickinson, Sparks, MD) to identify methicillin-resistant Staphylococcus aureus (MRSA) directly upon subculture from positive blood culture bottles. There were 124 MRSA isolates recovered from blood cultures in the study. BBL CHROMagar MRSA medium was highly sensitive (97.6% [121/124] at 18 to 24 h of incubation and 100% [124/124] at 48 h) and 99.9% specific for identifying MRSA from positive blood cultures.

* Corresponding author. Mailing address: Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, 4th Floor, Gates Building, 3400 Spruce St., Philadelphia, PA 19104-4283. Phone: (215) 662-6651. Fax: (215) 662-6655. E-mail: nachamki{at}mail.med.upenn.edu.

Journal of Clinical Microbiology, July 2006, p. 2575-2576, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00399-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bennett, K., Sharp, S. E. (2008). Rapid Differentiation of Methicillin-Resistant Staphylococcus aureus and Methicillin-Susceptible Staphylococcus aureus from Blood Cultures by Use of a Direct Cefoxitin Disk Diffusion Test. J. Clin. Microbiol. 46: 3836-3838 [Abstract] [Full Text]  
  • Baron, E. J., D'Souza, H., Qi Wang, A., Gibbs, D. L. (2008). Evaluation of the Biomic V3 Microbiology System for Identification of Selected Species on BBL CHROMagar Orientation Agar and CHROMagar MRSA Medium. J. Clin. Microbiol. 46: 3488-3490 [Abstract] [Full Text]  
  • Stamper, P. D., Cai, M., Howard, T., Speser, S., Carroll, K. C. (2007). Clinical Validation of the Molecular BD GeneOhm StaphSR Assay for Direct Detection of Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus in Positive Blood Cultures. J. Clin. Microbiol. 45: 2191-2196 [Abstract] [Full Text]  


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