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Journal of Clinical Microbiology, July 2006, p. 2601-2604, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.02282-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Use of 16S rRNA Gene Profiling by Terminal Restriction Fragment Length Polymorphism Analysis To Compare Bacterial Communities in Sputum and Mouthwash Samples from Patients with Cystic Fibrosis{dagger}

G. B. Rogers,1,2 M. P. Carroll,2 D. J. Serisier,2,4 P. M. Hockey,2 G. Jones,3 V. Kehagia,2 G. J. Connett,2 and K. D. Bruce1*

King's College London, Pharmaceutical Science Research Division, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, United Kingdom,1 Cystic Fibrosis Unit, Southampton University Hospitals NHS Trust, Southampton, United Kingdom,2 The Public Health Laboratory Service, Southampton University Hospitals, Southampton, United Kingdom,3 The Adult Cystic Fibrosis Unit, Mater Adult Hospital, Brisbane, Australia4

Received 1 November 2005/ Returned for modification 5 December 2005/ Accepted 26 April 2006

The bacterial communities present in the oral cavity and the lungs of 19 adult cystic fibrosis (CF) patients were compared by using terminal restriction fragment length polymorphism analysis of 16S rRNA gene PCR products amplified from nucleic acids extracted directly from bacteria in clinical samples. Sputum samples were not found to be subject to profound contamination by oral cavity bacteria. Evidence of colonization of the CF lung by certain oral bacterial species was found.

* Corresponding author. Mailing address: King's College London, Pharmaceutical Science Research Division, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, United Kingdom. Phone: 44 020 7848 4670. Fax: 44 020 7848 4500. E-mail: kenneth.bruce{at}kcl.ac.uk.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, July 2006, p. 2601-2604, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.02282-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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