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Journal of Clinical Microbiology, July 2006, p. 2612-2614, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00449-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Blinded, Multicenter Comparison of Methods To Detect a Drug-Resistant Mutant of Human Immunodeficiency Virus Type 1 at Low Frequency

Elias K. Halvas,1 Grace M. Aldrovandi,2 Peter Balfe,3 Ingrid A. Beck,4 Valerie F. Boltz,5 John M. Coffin,5 Lisa M. Frenkel,4 J. Darren Hazelwood,6 Victoria A. Johnson,6 Mary Kearney,5 Andrea Kovacs,7 Daniel R. Kuritzkes,8 Karin J. Metzner,9 Dwight V. Nissley,10 Marek Nowicki,7 Sarah Palmer,5 Rainer Ziermann,11 Richard Y. Zhao,12 Cheryl L. Jennings,13 James Bremer,13 Don Brambilla,14 and John W. Mellors1*

University of Pittsburgh, Pittsburgh, Pennsylvania,1 Children's Hospital of Los Angeles, Keck School of Medicine, Los Angeles, California,2 Columbia University, New York, New York,3 University of Washington, Seattle, Washington,4 HIV Drug Resistance Program, National Cancer Institute, Frederick, Maryland,5 Birmingham VA Medical Center and University of Alabama at Birmingham, Birmingham, Alabama,6 University of Southern California, Los Angeles, California,7 Section of Retroviral Therapeutics, Brigham and Women's Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts,8 University of Erlangen- Nuremberg, Erlangen, Germany,9 Basic Research Program, SAIC-Frederick, National Cancer Institute, Frederick, Maryland,10 Bayer HealthCare-Diagnostics, Berkeley, California,11 University of Maryland School of Medicine, Baltimore, Maryland,12 Rush Medical College, Chicago, Illinois,13 New England Research Institute, Watertown, Massachusetts,14

Received 1 March 2006/ Returned for modification 14 April 2006/ Accepted 25 April 2006

We determined the abilities of 10 technologies to detect and quantify a common drug-resistant mutant of human immunodeficiency virus type 1 (lysine to asparagine at codon 103 of the reverse transcriptase) using a blinded test panel containing mutant-wild-type mixtures ranging from 0.01% to 100% mutant. Two technologies, allele-specific reverse transcriptase PCR and a Ty1HRT yeast system, could quantify the mutant down to 0.1 to 0.4%. These technologies should help define the impact of low-frequency drug-resistant mutants on response to antiretroviral therapy.

* Corresponding author. Mailing address: Division of Infectious Diseases, Department of Medicine, University of Pittsburgh, S818 Scaife Hall, 3550 Terrace St., Pittsburgh, PA 15261. Phone: (412) 383-7963. Fax: (412) 687-7982. E-mail: Mellors{at}msx.dept-med.pitt.edu.

Journal of Clinical Microbiology, July 2006, p. 2612-2614, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00449-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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