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Journal of Clinical Microbiology, July 2006, p. 2626-2629, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00895-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Rapid Identification and Differentiation of Clinical Isolates of Enteropathogenic Escherichia coli (EPEC), Atypical EPEC, and Shiga Toxin-Producing Escherichia coli by a One-Step Multiplex PCR Method

Daniel Müller,1 Peter Hagedorn,1 Sabine Brast,1 Gerhard Heusipp,1 Martina Bielaszewska,2 Alexander W. Friedrich,2 Helge Karch,2 and M. Alexander Schmidt1*

Institut für Infektiologie, Zentrum für Molekularbiologie der Entzündung, Westfälische Wilhelms-Universität/ Universitätsklinikum Münster,1 Institut für Hygiene und Nationales Konsiliarlaboratorium für Hämolytisch-Urämisches Syndrom, Universitätsklinikum Münster, D-48149 Münster, Germany2

Received 28 April 2006/ Accepted 3 May 2006

Enteropathogenic Escherichia coli (EPEC), atypical enteropathogenic E. coli, and Shiga toxin-producing E. coli differ in their virulence factor profiles, clinical manifestations, and prognosis, and they require different therapeutic measures. We developed and evaluated a robust multiplex PCR to identify these pathogroups based on sequences complementary to escV, bfpB, stx1, and stx2.

* Corresponding author. Mailing address: Institut für Infektiologie, Zentrum für Molekularbiologie der Entzündung (ZMBE), Von-Esmarch-Str. 56, D-48149 Münster, Germany. Phone: 49 251 835 6466. Fax: 49 251 835 6467. E-mail: infekt{at}uni-muenster.de.

Journal of Clinical Microbiology, July 2006, p. 2626-2629, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00895-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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  • Bielaszewska, M., Stoewe, F., Fruth, A., Zhang, W., Prager, R., Brockmeyer, J., Mellmann, A., Karch, H., Friedrich, A. W. (2009). Shiga Toxin, Cytolethal Distending Toxin, and Hemolysin Repertoires in Clinical Escherichia coli O91 Isolates. J. Clin. Microbiol. 47: 2061-2066 [Abstract] [Full Text]  
  • Ewers, C., Antao, E.-M., Diehl, I., Philipp, H.-C., Wieler, L. H. (2009). Intestine and Environment of the Chicken as Reservoirs for Extraintestinal Pathogenic Escherichia coli Strains with Zoonotic Potential. Appl. Environ. Microbiol. 75: 184-192 [Abstract] [Full Text]  
  • Muller, D., Greune, L., Heusipp, G., Karch, H., Fruth, A., Tschape, H., Schmidt, M. A. (2007). Identification of Unconventional Intestinal Pathogenic Escherichia coli Isolates Expressing Intermediate Virulence Factor Profiles by Using a Novel Single-Step Multiplex PCR. Appl. Environ. Microbiol. 73: 3380-3390 [Abstract] [Full Text]  


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