This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fonseca, E. L.
Right arrow Articles by Rodrigues, D. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fonseca, E. L.
Right arrow Articles by Rodrigues, D. P.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2006, p. 2767-2772, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.01916-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Clonality and Antimicrobial Resistance Gene Profiles of Multidrug- Resistant Salmonella enterica Serovar Infantis Isolates from Four Public Hospitals in Rio de Janeiro, Brazil

E. L. Fonseca,1 O. L. Mykytczuk,4,5 M. D. Asensi,1 E. M. F. Reis,1 L. R. Ferraz,2 F. L. Paula,3 L. K. Ng,4,5 and D. P. Rodrigues1*

Bacteriology Department, Oswaldo Cruz Institute - FIOCRUZ, Rio de Janeiro, Brazil,1 Public Health Laboratory, Brasilia, Brazil,2 Evandro Chagas Institute, Para, Brazil,3 National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada,4 Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada5

Received 13 September 2005/ Returned for modification 7 November 2005/ Accepted 8 May 2006

In Brazil, Salmonella enterica serovar Infantis resistant to various antimicrobials, including cephalosporins, has been identified as an etiological agent of severe gastroenteritis in hospitalized children since 1994. In this study, 35 serovar Infantis strains, isolated from children admitted to four different Rio de Janeiro, Brazil, hospitals between 1996 and 2001, were characterized by pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing in order to determine their genetic relatedness and antimicrobial resistance profiles. Thirty-four serovar Infantis strains were resistant to at least two antibiotic classes, and all 35 strains were susceptible to fluoroquinolones, cephamycin, and carbapenem. Extended-spectrum beta-lactamase (ESBL) screening by double-disk diffusion indicated that 32 serovar Infantis strains (91.4%) produced beta-lactamases that were inhibited by clavulanic acid. Antimicrobial resistance gene profiles were determined by PCR for a subset of 11 multidrug-resistant serovar Infantis strains, and putative ESBLs were detected by isoelectric focusing. Ten serovar Infantis strains carried blaTEM, catI, ant(3")Ia and/or ant(3")Ib, sulI and/or sulII, and tet(D) genes as well as an integron-associated aac(6')-Iq cassette. Eight strains possessed at least four different beta-lactamases with pI profiles that confirmed the presence of both ESBLs and non-ESBLs. Our PFGE profiles indicated that 33 serovar Infantis strains isolated from Rio de Janeiro hospitals came from the same genetic lineage.

* Corresponding author. Mailing address: Laboratório de Enterobactérias, Departamento de Bacteriologia, Oswaldo Cruz Institute - FIOCRUZ, Avenida Brasil, 4365 - Pavilhão Rocha Lima, 3° andar, Manguinhos - Rio de Janeiro, Brasil 21040-361. Phone: 55 21 2598 4277. Fax: 55 21 2270 6565. E-mail: dalia{at}ioc.fiocruz.br.

Journal of Clinical Microbiology, August 2006, p. 2767-2772, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.01916-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lynne, A. M., Rhodes-Clark, B. S., Bliven, K., Zhao, S., Foley, S. L. (2008). Antimicrobial Resistance Genes Associated with Salmonella enterica Serovar Newport Isolates from Food Animals. Antimicrob. Agents Chemother. 52: 353-356 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»