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Journal of Clinical Microbiology, August 2006, p. 2808-2815, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.00048-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Typing of Human Adenoviruses in Specimens from Immunosuppressed Patients by PCR-Fragment Length Analysis and Real-Time Quantitative PCR

Karin Ebner, Margit Rauch, Sandra Preuner, and Thomas Lion*

Division of Molecular Microbiology and Development of Genetic Diagnostics, Children's Cancer Research Institute, A-1090 Vienna, Austria

Received 10 January 2006/ Returned for modification 14 March 2006/ Accepted 26 May 2006

Currently, 51 human adenovirus (AdV) serotypes, which are divided into six species (A to F), are known. AdV infections are a major cause of morbidity and mortality in immunosuppressed individuals, particularly in allogeneic stem cell transplant (SCT) recipients. Any AdV species may cause life-threatening disease, but little information is available on the clinical relevance of individual serotypes. The use of serological testing for serotype identification is limited due to the impaired immune response during the posttransplant period. A new molecular approach to serotype identification is presented here that exploits variable regions within the hexon gene. All serotypes belonging to the species A, B, C, E, and F can be determined by fragment length analysis of a single PCR product. For species C, which is the most prevalent in many geographic regions, an alternative technique based on serotype-specific real-time quantitative PCR was established. Of 135 consecutive pediatric patients screened for AdV infections after allogeneic SCT, 40 tested positive. Detailed analysis revealed the presence of 10 different serotypes; serotypes 1 and 2 from species C (C01 and C02) showed the highest prevalence, accounting for 77% of the AdV-positive cases. Representatives of other species were observed less commonly: serotype A12 in 6.5%; serotype A31 in 4.5%; and B03, B16, C05, C06, D19, and F41 in 2%. The approach to rapid molecular serotype analysis presented here provides a basis for detailed studies on adenovirus epidemiology and on the transmission of nosocomial infections. Moreover, in view of the increasing importance of tailored therapy approaches, serotype identification may in the future have implications for the selection of the most appropriate antiviral treatment.

* Corresponding author. Mailing address: CCRI, Kinderspitalgasse 6, A-1090 Vienna, Austria. Phone: 43-[0]1-40470-489. Fax: 43-[0]1-40470-437. E-mail: Thomas.Lion{at}ccri.at.

Journal of Clinical Microbiology, August 2006, p. 2808-2815, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.00048-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zheng, X., Lu, X., Erdman, D. D., Anderson, E. J., Guzman-Cottrill, J. A., Kletzel, M., Katz, B. Z. (2008). Identification of Adenoviruses in Specimens from High-Risk Pediatric Stem Cell Transplant Recipients and Controls. J. Clin. Microbiol. 46: 317-320 [Abstract] [Full Text]  
  • Kosulin, K., Haberler, C., Hainfellner, J. A., Amann, G., Lang, S., Lion, T. (2007). Investigation of Adenovirus Occurrence in Pediatric Tumor Entities. J. Virol. 81: 7629-7635 [Abstract] [Full Text]  


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