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Journal of Clinical Microbiology, August 2006, p. 2983-2987, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.02544-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Comparison of Real-Time PCR Assays for Monitoring Serum Hepatitis B Virus DNA Levels during Antiviral Therapy

Chee-Kin Hui,1,2,3 Scott Bowden,4 Hai-Ying Zhang,1,2 Anita Wong,5 Sharon Lewin,6,7 Frank Rousseau,8 Herve Mommeja-Marin,8 Nikki P. Lee,2,9 John M. Luk,2,9 Stephen Locarnini,4 Nancy Leung,10 Nikolai V. Naoumov,11 and George K. K. Lau1,2,3*

Department of Medicine, The University of Hong Kong, Hong Kong SAR, China,1 Centre for the Study of Liver Diseases, The University of Hong Kong, Hong Kong SAR, China,2 Research Centre for Infection and Immunity, The University of Hong Kong, Hong Kong SAR, China,3 Victorian Infectious Diseases Reference Laboratory, North Melbourne, Victoria, Australia,4 Clinical Trials Centre, The University of Hong Kong, Hong Kong SAR, China,5 Victorian Infectious Diseases Service, The Royal Melbourne Hospital, Parkville, Victoria, Australia,6 Department of Microbiology and Immunology, The University of Melbourne, Parkville, Victoria, Australia,7 Gilead Sciences Inc., Durham, North Carolina,8 Department of Surgery, The University of Hong Kong, Hong Kong SAR, China,9 Department of Medicine, AHML Nethersole Hospital, Hong Kong SAR, China,10 Institute of Hepatology, University College London, London, United Kingdom,11

Received 7 December 2005/ Returned for modification 12 April 2006/ Accepted 23 May 2006

The performance characteristics of the RealART and Molecular Beacons assays were compared with those of the Digene Hybrid Capture II assay (ultrasensitive). The results of the RealART and Digene Hybrid assays were related (r = 0.94; P < 0.001) and diverged by 2 orders of magnitude. The RealART assay can be used to effectively monitor serum hepatitis B virus DNA levels.

* Corresponding author. Mailing address: Department of Medicine, University of Hong Kong, Queen Mary Hospital, Room 1838, Block K, 102 Pokfulam Road, Hong Kong, SAR, China. Phone: (852) 28553578. Fax: (852) 28190694. E-mail: gkklau{at}netvigator.com.

Journal of Clinical Microbiology, August 2006, p. 2983-2987, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.02544-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Valsamakis, A. (2007). Molecular Testing in the Diagnosis and Management of Chronic Hepatitis B. Clin. Microbiol. Rev. 20: 426-439 [Abstract] [Full Text]  


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