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Journal of Clinical Microbiology, August 2006, p. 3025-3027, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.00068-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Evaluation of the GenoType Mycobacteria Direct Assay for Detection of Mycobacterium tuberculosis Complex and Four Atypical Mycobacterial Species in Clinical Samples

F. Franco-Álvarez de Luna, P. Ruiz, J. Gutiérrez, and M. Casal*

Microbiology Service, Reina Sofia University Hospital, and Mycobacteria Reference Center, Faculty of Medicine, Cordoba University, Cordoba, Spain

Received 12 January 2006/ Returned for modification 7 March 2006/ Accepted 2 June 2006

We evaluated GenoType Mycobacteria Direct (GTMD), a novel commercial assay based on nucleic acid sequence-based amplification technology, for the detection of Mycobacterium tuberculosis complex, M. avium, M. intracellulare, M. kansasii, and M. malmoense directly from clinical specimens. A total of 134 respiratory and extrapulmonary samples from 65 patients were processed. Sensitivity, specificity, positive predictive, and negative predictive values for GTMD were 92, 100, 100, and 77%, respectively. The GMTD technique is useful, reliable, and rapid when used during the normal routine of a clinical laboratory.

* Corresponding author. Mailing address: Mycobacteria Reference Center, Faculty of Medicine, Cordoba University, 14004 Cordoba, Spain. Phone: 34 957 218284. Fax: 34 957 218229. E-mail: mi1carom{at}uco.es.

Journal of Clinical Microbiology, August 2006, p. 3025-3027, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.00068-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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