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Journal of Clinical Microbiology, August 2006, p. 3028-3030, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.00913-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Clinical Evaluation of a Type III Secretion System Real-Time PCR Assay for Diagnosing Melioidosis

Ella M. Meumann,1 Ryan T. Novak,2 Daniel Gal,1 Mirjam E. Kaestli,1 Mark Mayo,1 Joshua P. Hanson,3 Emma Spencer,3 Mindy B. Glass,2 Jay E. Gee,2 Patricia P. Wilkins,2 and Bart J. Currie1,3*

Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia,1 Bacterial Zoonoses Branch, Division of Foodborne, Bacterial, and Mycotic Diseases, National Center for Zoonotic, Vectorborne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333,2 Infectious Diseases Unit, Royal Darwin Hospital and Northern Territory Clinical School, Darwin, Northern Territory, Australia3

Received 2 May 2006/ Returned for modification 25 May 2006/ Accepted 15 June 2006

A Burkholderia pseudomallei type III secretion system real-time PCR assay was evaluated on clinical specimens in a region where melioidosis is endemic. The PCR was positive in 30/33 (91%) patients with culture-confirmed melioidosis. All six patients with melioidosis septic shock were blood PCR positive, suggesting potential for rapid diagnosis and commencement of appropriate therapy.

* Corresponding author. Mailing address: Menzies School of Health Research, P.O. Box 41096 Casuarina, Northern Territory 0811, Australia. Phone: 61-8-89228196. Fax: 61-8-89275187. E-mail: bart{at}menzies.edu.au.

Journal of Clinical Microbiology, August 2006, p. 3028-3030, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.00913-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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