This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yu, F.
Right arrow Articles by Morita, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yu, F.
Right arrow Articles by Morita, K.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2006, p. 3134-3138, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00693-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Serodiagnosis Using Recombinant Nipah Virus Nucleocapsid Protein Expressed in Escherichia coli

Fuxun Yu,1 Nor Shahidah Khairullah,2 Shingo Inoue,1 Vijayamalar Balasubramaniam,2 Stella Joan Berendam,2 Leok Kin Teh,2 Nik Shamsiah Wan Ibrahim,2 Sohayati Abdul Rahman,3 Sharifah Syed Hassan,3 Futoshi Hasebe,1 Mangalam Sinniah,2,{dagger} and Kouichi Morita1*

Department of Virology, Institute of Tropical Medicine, Nagasaki University, 1-12-4, Sakamoto, Nagasaki 852-8523, Japan,1 Department of Virology, Institute for Medical Research, Kuala Lumpur,2 Veterinary Research Institute, Ipoh, Malaysia3

Received 3 April 2006/ Returned for modification 12 June 2006/ Accepted 20 June 2006

Nipah virus nucleocapsid (NiV-N) protein was expressed in Escherichia coli and purified by histidine tag-based affinity chromatography. An indirect immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) for human and swine sera and an IgM capture ELISA for human sera were established using the recombinant NiV-N protein as an antigen. One hundred thirty-three suspected patient sera and 16 swine sera were used to evaluate the newly established ELISA systems in comparison with the CDC inactivated-virus-based ELISA systems. For the human sera, the NiV-N protein-based indirect IgG ELISA had a sensitivity of 98.6% and a specificity of 98.4%, and the NiV-N protein-based IgM capture ELISA had a sensitivity of 91.7% and a specificity of 91.8%, with reference to the CDC ELISA systems. The NiV-N-based IgM ELISA was found to be more sensitive than the inactivated-virus-based ELISA in that it captured eight additional cases. For the swine sera, the two test systems were in 100% concordance. Our data indicate that the Nipah virus nucleocapsid protein is a highly immunogenic protein in human and swine infections and a good target for serodiagnosis. Our NiV-N protein-based ELISA systems are useful, safe, and affordable tools for diagnosis of Nipah virus infection and are especially fit to be used in large-scale epidemiological investigations and to be applied in developing countries.

* Corresponding author. Mailing address: Department of Virology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan. Phone: 81 95 849 7829. Fax: 81 95 849 7830. E-mail: moritak{at}net.nagasaki-u.ac.jp.

{dagger} Present address: Department of Pathology, Kuala Lumpur Hospital, Kuala Lumpur, Malaysia.

Journal of Clinical Microbiology, September 2006, p. 3134-3138, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00693-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»