This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nagarajan, T.
Right arrow Articles by Srinivasan, V. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nagarajan, T.
Right arrow Articles by Srinivasan, V. A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2006, p. 3218-3224, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00801-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Molecular Epidemiology of Rabies Virus Isolates in India

T. Nagarajan,1 B. Mohanasubramanian,1 E. V. Seshagiri,1 S. B. Nagendrakumar,1 M. R. Saseendranath,2 M. L. Satyanarayana,3 D. Thiagarajan,1 P. N. Rangarajan,4 and V. A. Srinivasan1*

Indian Immunologicals Limited, Gachibowli, Hyderabad 500 032, India,1 College of Veterinary Sciences, Mannuthy, Thrissur 680 651, India,2 Veterinary College, KVASSU, Hebbal, Bangalore 560 024, India,3 Indian Institute of Science, Bangalore, 560 012, India4

Received 14 April 2006/ Returned for modification 26 May 2006/ Accepted 19 June 2006

In India, rabies is enzootic and is a serious public health and economic problem. India has a large population of stray dogs which, together with a lack of effective control strategies, might have led to the persistence of rabies virus (RV) in the canine population. Our objective was to study the molecular epidemiology of RV isolates in India based on nucleotide sequence analysis of 29 RV isolates originating from different species of animals in four states. Here we have analyzed two sets of sequence data based upon a 132-nucleotide region of the cytoplasmic domain (CD) of the G gene (G-CD) and a 549-nucleotide region (Psi-L) that combines the noncoding G-L intergenic region (Psi) and a fragment of the polymerase gene (L). Phylogenetic analysis revealed that the RV isolates belong to genotype 1 and that they were related geographically but were not related according to host species. Five different genetic clusters distributed among three geographical regions were identified. Comparison of the deduced amino acid sequences of G-CD between RV isolates revealed three amino acid changes (amino acid 462G [aa462G], aa465H, and aa468K) that distinguished the Indian RVs from RV isolates in other parts of the world. Analysis of the data indicated that the dog rabies virus variants are the major circulating viruses in India that transmit the disease to other domestic animals and humans as well.

* Corresponding author: Mailing address: Indian Immunologicals Limited, Gachibowli, Hyderabad 500 032, India. Phone: 91-40-23000894. Fax: 91-40-23005958. E-mail: srini{at}indimmune.com.

Journal of Clinical Microbiology, September 2006, p. 3218-3224, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00801-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»