This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lu, X.
Right arrow Articles by Erdman, D. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lu, X.
Right arrow Articles by Erdman, D. D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2006, p. 3231-3235, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00889-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Real-Time PCR Assays for Detection of Bocavirus in Human Specimens

Xiaoyan Lu,1 Malinee Chittaganpitch,2 Sonja J. Olsen,3 Ian M. Mackay,4 Theo P. Sloots,4 Alicia M. Fry,5 and Dean D. Erdman1*

Respiratory and Gastroenteritis Viruses Branch,1 Epidemiology Branch, Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia,5 National Institute of Health, Thailand Ministry of Public Health, Nonthaburi, Thailand,2 International Emerging Infections Program, Thai MOPH-U.S. CDC Collaboration, Nonthaburi, Thailand,3 Qpid Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital and Clinical Medical Virology Centre, University of Queensland, Queensland, Australia4

Received 27 April 2006/ Returned for modification 6 July 2006/ Accepted 17 July 2006

The recently discovered human bocavirus (HBoV) is the first member of the family Parvoviridae, genus Bocavirus, to be potentially associated with human disease. Several studies have identified HBoV in respiratory specimens from children with acute respiratory disease, but the full spectrum of clinical disease and the epidemiology of HBoV infection remain unclear. The availability of rapid and reliable molecular diagnostics would therefore aid future studies of this novel virus. To address this, we developed two sensitive and specific real-time TaqMan PCR assays that target the HBoV NS1 and NP-1 genes. Both assays could reproducibly detect 10 copies of a recombinant DNA plasmid containing a partial region of the HBoV genome, with a dynamic range of 8 log units (101 to 108 copies). Eight blinded clinical specimen extracts positive for HBoV by an independent PCR assay were positive by both real-time assays. Among 1,178 NP swabs collected from hospitalized pneumonia patients in Sa Kaeo Province, Thailand, 53 (4.5%) were reproducibly positive for HBoV by one or both targets. Our data confirm the possible association of HBoV infection with pneumonia and demonstrate the utility of these real-time PCR assays for HBoV detection.

* Corresponding author. Mailing address: Respiratory and Gastroenteritis Viruses Branch, Centers for Disease Control and Prevention, 1600 Clifton Road, N.E., Mailstop G04, Atlanta, GA 30333. Phone: (404) 639-3727. Fax: (404) 639-4416. E-mail: dde1{at}cdc.gov.

Journal of Clinical Microbiology, September 2006, p. 3231-3235, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00889-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cecchini, S., Negrete, A., Virag, T., Graham, B. S., Cohen, J. I., Kotin, R. M. (2009). Evidence of Prior Exposure to Human Bocavirus as Determined by a Retrospective Serological Study of 404 Serum Samples from Adults in the United States. CVI 16: 597-604 [Abstract] [Full Text]  
  • Mahony, J. B. (2008). Detection of Respiratory Viruses by Molecular Methods. Clin. Microbiol. Rev. 21: 716-747 [Abstract] [Full Text]  
  • Schildgen, O., Muller, A., Allander, T., Mackay, I. M., Volz, S., Kupfer, B., Simon, A. (2008). Human Bocavirus: Passenger or Pathogen in Acute Respiratory Tract Infections?. Clin. Microbiol. Rev. 21: 291-304 [Abstract] [Full Text]  
  • Lu, X., Holloway, B., Dare, R. K., Kuypers, J., Yagi, S., Williams, J. V., Hall, C. B., Erdman, D. D. (2008). Real-Time Reverse Transcription-PCR Assay for Comprehensive Detection of Human Rhinoviruses. J. Clin. Microbiol. 46: 533-539 [Abstract] [Full Text]  
  • Endo, R., Ishiguro, N., Kikuta, H., Teramoto, S., Shirkoohi, R., Ma, X., Ebihara, T., Ishiko, H., Ariga, T. (2007). Seroepidemiology of Human Bocavirus in Hokkaido Prefecture, Japan. J. Clin. Microbiol. 45: 3218-3223 [Abstract] [Full Text]  
  • Neske, F., Blessing, K., Tollmann, F., Schubert, J., Rethwilm, A., Kreth, H. W., Weissbrich, B. (2007). Real-Time PCR for Diagnosis of Human Bocavirus Infections and Phylogenetic Analysis. J. Clin. Microbiol. 45: 2116-2122 [Abstract] [Full Text]  
  • Kleines, M., Scheithauer, S., Rackowitz, A., Ritter, K., Hausler, M. (2007). High Prevalence of Human Bocavirus Detected in Young Children with Severe Acute Lower Respiratory Tract Disease by Use of a Standard PCR Protocol and a Novel Real-Time PCR Protocol. J. Clin. Microbiol. 45: 1032-1034 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»