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Journal of Clinical Microbiology, September 2006, p. 3257-3262, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00433-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Evaluation of the New VITEK 2 Extended-Spectrum Beta-Lactamase (ESBL) Test for Rapid Detection of ESBL Production in Enterobacteriaceae Isolates

Teresa Spanu,^1* Maurizio Sanguinetti,¹ Mario Tumbarello,² Tiziana D'Inzeo,¹ Barbara Fiori,¹ Brunella Posteraro,¹ Rosaria Santangelo,¹ Roberto Cauda,² and Giovanni Fadda¹

Institute of Microbiology,¹ Department of Infectious Diseases, Catholic University of the Sacred Heart, Rome, Italy²

Received 28 February 2006/ Returned for modification 23 March 2006/ Accepted 7 July 2006

Extended-spectrum beta-lactamases (ESBLs) are a large, rapidly evolving group of enzymes that confer resistance to oxyimino cephalosporins and monobactams and are inhibited by clavulanate. Rapid reliable detection of ESBL production is a prerequisite for successful infection management and for monitoring resistance trends and implementation of intervention strategies. We evaluated the performance of the new VITEK 2 ESBL test system (bioMérieux, Inc, Hazelwood, Mo.) in the identification of ESBL-producing Enterobacteriaceae isolates. We examined a total of 1,129 clinically relevant Enterobacteriaceae isolates (including 218 that had been previously characterized). The ESBL classification furnished by the VITEK 2 ESBL test system was concordant with that of the comparison method (molecular identification of beta-lactamase genes) for 1,121 (99.3%) of the 1,129 isolates evaluated. ESBL production was correctly detected in 306 of the 312 ESBL-producing organisms (sensitivity, 98.1%; positive predictive value, 99.3%). False-positive results emerged for 2 of the 817 ESBL-negative isolates (specificity, 99.7%; negative predictive value, 99.3%). VITEK 2 ESBL testing took 6 to 13 h (median, 7.5 h; mean ± SD, 8.2 ± 2.39 h). This automated short-incubation system appears to be a rapid and reliable tool for routine identification of ESBL-producing isolates of Enterobacteriaceae.

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* Corresponding author. Mailing address: Istituto Microbiologia, Università Cattolica del Sacro Cuore, Largo A. Gemelli 8, 00168 Roma, Italy. Phone: 39-06-30154218. Fax: 39-06-3051152. E-mail: tspanu{at}rm.unicatt.it.

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Journal of Clinical Microbiology, September 2006, p. 3257-3262, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00433-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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