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Journal of Clinical Microbiology, September 2006, p. 3299-3305, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00417-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

High-Throughput Identification of Clinical Pathogenic Fungi by Hybridization to an Oligonucleotide Microarray{dagger}

Aihua Huang,1,2 Jun-Wen Li,1* Zhi-Qiang Shen,1 Xin-Wei Wang,1 and Min Jin1

Institute of Environment and Health, No. 1, Dali Road, Tianjin, 300050,1 Medical College of Chinese People's Armed Police Forces, Tianjin, 300162, People's Republic of China2

Received 26 February 2006/ Returned for modification 11 April 2006/ Accepted 6 June 2006

Here we report the development of an oligonucleotide microarray method that can identify fungal pathogens in a single reaction. Specific oligonucleotide probes targeted to internal transcribed spacer 2 were designed and synthesized. Fungal DNA was amplified by universal primers, and the PCR product was hybridized with the oligonucleotide microarray. A series of specific hybridization profiles corresponding to species were obtained. The 122 strains of fungal pathogens, including standard and clinically isolated strains, used to test the specificity, stability, and sensitivity of the microarray system belonged to 20 species representing 8 genera. We found that the microarray system can successfully discriminate among the fungal pathogens to the species level, with high specificity and stability. The sensitivity was 15 pg/ml of DNA. This oligonucleotide microarray system represents a rapid, simple, and reliable alternative to conventional methods of identifying common clinical fungal isolates.

* Corresponding author. Mailing address: Department of Environment and Health, Tianjin Institute of Environment and Health, 1 Dali Road, Tianjin 300050, People's Republic of China. Phone: 86-22-84655345. Fax: 86-22-23328809. E-mail: junwen9999{at}hotmail.com.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, September 2006, p. 3299-3305, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00417-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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