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Journal of Clinical Microbiology, September 2006, p. 3394-3396, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00493-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Diagnostic Value of Reverse Transcription-PCR for Detection of Cytomegalovirus pp67 in Samples from Solid-Organ Transplant Recipients

Ursula Meyer-Koenig,1* Ilona Romberg,1 Kerstin Schneider,1 Manfred Weidmann,2 Winfried V. Kern,3 and Frank T. Hufert2

Department of Virology, Institute for Medical Microbiology, University Hospital Freiburg, Hermann-Herder-Str. 11, D 79104 Freiburg, Germany,1 Institute for Virology, University of Goettingen, Kreuzbergring 57, D 37075 Goettingen, Germany,2 Department of Medicine, University Hospital Freiburg, Hugstetterstr. 49, D 79095 Freiburg, Germany3

Received 7 March 2006/ Returned for modification 25 April 2006/ Accepted 3 July 2006

We evaluated a highly sensitive quantitative real-time one-step reverse transcription-PCR (RT-PCR) for detection of human cytomegalovirus pp67 transcripts in monitoring of solid-organ transplant recipients. Results were compared with those of pp65 antigen testing and quantitative DNA-PCR. Due to a low clinical sensitivity, the pp67 RT-PCR was not able to replace these assays.

* Corresponding author. Mailing address: Department of Virology, Institute for Medical Microbiology, University Hospital Freiburg, Hermann-Herder-Str. 11, D 79104 Freiburg, Germany. Phone: 49-761-203-6611. Fax: 49-761-203-6608. E-mail: ursula.meyer-koenig{at}uniklinik-freiburg.de.

Journal of Clinical Microbiology, September 2006, p. 3394-3396, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00493-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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