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Journal of Clinical Microbiology, September 2006, p. 3397-3400, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00247-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Differential In Vitro Expression of the brkA Gene in Bordetella pertussis and Bordetella parapertussis Clinical Isolates

Paola Stefanelli,1 Maurizio Sanguinetti,2 Cecilia Fazio,1 Brunella Posteraro,2 Giovanni Fadda,2 and Paola Mastrantonio1*

Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy,1 Institute of Microbiology, Università Cattolica del Sacro Cuore, Rome, Italy2

Received 3 February 2006/ Returned for modification 13 March 2006/ Accepted 17 July 2006

In this study, we set up a real-time reverse transcriptase PCR assay to measure the relative amounts of brkA transcripts in 50 Bordetella isolates. The results suggested that brkA expression is strain dependent and its level may play a role in determining the serum resistance or susceptibility phenotype. Pertussis immunocompetent sera were unable to kill Bordetella parapertussis via complement deposition.

* Corresponding author. Mailing address: Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, V. le Regina Elena 299, 00161 Rome, Italy. Phone: 390649902335. Fax: 390649387112. E-mail: pmastran{at}iss.it.

Journal of Clinical Microbiology, September 2006, p. 3397-3400, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00247-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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