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Journal of Clinical Microbiology, September 2006, p. 3429-3431, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00989-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

A Modified Christensen's Urea and CLSI Broth Microdilution Method for Testing Susceptibilities of Six Malassezia Species to Voriconazole, Itraconazole, and Ketoconazole

S. Rincón,1 M. C. Cepero de García,1 and A. Espinel-Ingroff2*

Centro De Investigaciones Microbiológicas CIMIC, Universidad de los Andes, Bogotá, Colombia,1 VCU Medical Center, Richmond, Virginia 23298-00492

Received 11 May 2006/ Returned for modification 19 June 2006/ Accepted 3 July 2006

Two supplemented broths (Christensen's urea with 0.1% Tween 80 and 0.5% Tween 40 and RPMI 1640 with 1% glycerol, 1% peptone, 1.8% glucose, and 0.05% Tween 80) were evaluated to determine voriconazole, itraconazole, and ketoconazole MICs for 200 Malassezia sp. isolates. Malassezia globosa and M. restricta were the least susceptible species (MICs at which 90% of the isolates tested were inhibited, 1 to ≥8 µg/ml versus 0.25 to 1 µg/ml).

* Corresponding author. Mailing address: VCU Medical Center, Medical Mycology Research Laboratory, 1101 E. Marshal Street, Sanger Hall Room 7049, P.O. Box 980049, Richmond, VA 23298-0049. Phone: (804) 828-5743. Fax: (804) 828-3097. E-mail: avingrof{at}vcu.edu.

Journal of Clinical Microbiology, September 2006, p. 3429-3431, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00989-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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