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Journal of Clinical Microbiology, January 2007, p. 159-167, Vol. 45, No. 1
0095-1137/07/$08.00+0     doi:10.1128/JCM.01322-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

IS1301 Fingerprint Analysis of Neisseria meningitidis Strains Belonging to the ET-15 Clone{triangledown}

Johannes Elias* and Ulrich Vogel

Institute for Hygiene and Microbiology, University of Würzburg, Würzburg, Germany

Received 27 June 2006/ Returned for modification 16 August 2006/ Accepted 30 October 2006

Meningococci of the ET-15 clone frequently cause clusters of invasive meningococcal disease (IMD) and are associated with a high case-fatality ratio. Timely typing of strains from outbreaks of IMD caused by this clone is hampered by the low variability of its surface antigens. We present a new Southern blot-based typing method for ET-15 meningococci based on the insertion element IS1301, which was present in all 70 ET-15 strains tested. Fingerprints were stable in vitro over a period of 100 days of cultivation on agar plates. The discriminatory power of IS1301 fingerprinting exceeded that of typing by serogrouping and antigen sequencing of the outer membrane proteins PorA and FetA, as determined by the analysis of 52 epidemiologically unrelated strains. In addition, the method provided conclusive results with regard to the comparison of strains from clusters of IMD. The investigation of insertion sites of IS1301 revealed several new intragenic insertions, among others, into open reading frames homologous to mafB and tspB. A previously described insertion in nadA was present in more than two-thirds of the strains analyzed, suggesting that NadA is probably an unreliable vaccine candidate for the prevention of ET-15 disease.

* Corresponding author. Mailing address: Institute for Hygiene and Microbiology, University of Würzburg, Josef-Schneider-Str. 2, Building E1, 97080 Würzburg, Germany. Phone: 49 931 20146905. Fax: 49 931 20146445. E-mail: jelias{at}hygiene.uni-wuerzburg.de.

{triangledown} Published ahead of print on 8 November 2006.

Journal of Clinical Microbiology, January 2007, p. 159-167, Vol. 45, No. 1
0095-1137/07/$08.00+0     doi:10.1128/JCM.01322-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lucidarme, J., Comanducci, M., Findlow, J., Gray, S. J., Kaczmarski, E. B., Guiver, M., Kugelberg, E., Vallely, P. J., Oster, P., Pizza, M., Bambini, S., Muzzi, A., Tang, C. M., Borrow, R. (2009). Characterization of fHbp, nhba (gna2132), nadA, porA, Sequence Type (ST), and Genomic Presence of IS1301 in Group B Meningococcal ST269 Clonal Complex Isolates from England and Wales. J. Clin. Microbiol. 47: 3577-3585 [Abstract] [Full Text]  
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