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Journal of Clinical Microbiology, January 2007, p. 224-226, Vol. 45, No. 1
0095-1137/07/$08.00+0     doi:10.1128/JCM.01893-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Rapid Molecular Strategy for Filovirus Detection and Characterization{triangledown}

Junhui Zhai,1,{dagger} Gustavo Palacios,1,{dagger} Jonathan S. Towner,2 Omar Jabado,1 Vishal Kapoor,1 Marietjie Venter,3 Allen Grolla,4 Thomas Briese,1 Janusz Paweska,5 Robert Swanepoel,5 Heinz Feldmann,4,6 Stuart T. Nichol,2 and W. Ian Lipkin1*

Jerome L. and Dawn Greene Infectious Disease Laboratory, Mailman School of Public Health, Greene Infectious Disease Laboratory, New York, New York,1 Special Pathogens Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia,2 Department of Medical Virology, University of Pretoria/NHLS Tshwane Academic Division, Pretoria, South Africa,3 Special Pathogens Unit, National Institute for Communicable Diseases, Sandringham, South Africa,4 Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada,5 Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada6

Received 11 September 2006/ Returned for modification 7 October 2006/ Accepted 11 October 2006

Filoviruses have the capacity to cause lethal outbreaks of hemorrhagic fever in primates. Here we present a simple consensus reverse transcription-PCR method for filovirus recognition and characterization and demonstrate its utility with all known filovirus strains. Phylogenetic assignment is achieved by automated web-based sequence analysis of amplification products.

* Corresponding author. Mailing address: Jerome L. and Dawn Greene Infectious Disease Laboratory, Mailman School of Public Health, Columbia University, 722 West 168th St., Rm. 1801, New York, NY 10032. Phone: (212) 342-9031. Fax: (212) 342-9044. E-mail: wil2001{at}columbia.edu.

{triangledown} Published ahead of print on 1 November 2006.

{dagger} J.Z. and G.P. contributed equally to this study.

Journal of Clinical Microbiology, January 2007, p. 224-226, Vol. 45, No. 1
0095-1137/07/$08.00+0     doi:10.1128/JCM.01893-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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