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Journal of Clinical Microbiology, January 2007, p. 244-245, Vol. 45, No. 1
0095-1137/07/$08.00+0     doi:10.1128/JCM.01131-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Comparison between Nasal Swabs and Nasopharyngeal Aspirates for, and Effect of Time in Transit on, Isolation of Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, and Moraxella catarrhalis{triangledown}

Kylie S. Carville,1,# Jacinta M. Bowman,2 Deborah Lehmann,1 and Thomas V. Riley2,3*

Telethon Institute for Child Health Research, Centre for Child Health Research, The University of Western Australia, Western Australia, Australia,1 Division of Microbiology & Infectious Diseases, PathWest Laboratory Medicine, Western Australia, Australia,2 Microbiology & Immunology, The University of Western Australia, Western Australia, Australia3

Received 2 June 2006/ Returned for modification 26 July 2006/ Accepted 18 October 2006

We assessed the impact of the use of nasal swabs or nasopharyngeal aspirates and the time from specimen collection to storage at –70°C on bacterial isolation. Haemophilus influenzae was isolated significantly less often from swabs than from nasopharyngeal aspirates. Samples in transit for >3 days were half as likely to grow Streptococcus pneumoniae and H. influenzae as those in transit for ≤3 days. There was no statistically significant difference for either Moraxella catarrhalis or Staphylococcus aureus.

* Corresponding author. Mailing address: Microbiology & Immunology, The University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands 6009 WA, Australia. Phone: 61-8-9346-3690. Fax: 61-8-9346-2912. E-mail: triley{at}cyllene.uwa.edu.au.

{triangledown} Published ahead of print on 1 November 2006.

# Present address: Centre for International Child Health, Murdoch Childrens Research Institute, Victoria, Australia.

Journal of Clinical Microbiology, January 2007, p. 244-245, Vol. 45, No. 1
0095-1137/07/$08.00+0     doi:10.1128/JCM.01131-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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