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Journal of Clinical Microbiology, January 2007, p. 257-258, Vol. 45, No. 1
0095-1137/07/$08.00+0     doi:10.1128/JCM.01552-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Use of Partial 16S rRNA Gene Sequencing for Identification of Legionella pneumophila and Non-pneumophila Legionella spp.{triangledown}

D. A. Wilson,1 U. Reischl,2 G. S. Hall,1 and G. W. Procop3*

The Cleveland Clinic, Cleveland, Ohio,1 University of Regensburg, Regensburg, Germany,2 The University of Miami, Miami, Florida3

Received 26 July 2006/ Returned for modification 11 September 2006/ Accepted 6 November 2006

We examined 49 Legionella species, 26 L. pneumophila and 23 non-pneumophila Legionella spp., using partial 16S rRNA gene sequencing. This approach accurately identified all the L. pneumophila isolates, characterized all non-pneumophila Legionella isolates as such within this genus, and classified most (20/23; 87%) of the non-pneumophila Legionella isolates to the species level.

* Corresponding author. Mailing address: University of Miami Miller School of Medicine, Department of Pathology (R-5), 1611 NW 112th Avenue, Holtz Center, 2090, Miami, FL 33136. Phone: (305) 585-5068. Fax: (305) 326-9363. E-mail: gprocop{at}med.miami.edu.

{triangledown} Published ahead of print on 15 November 2006.

Journal of Clinical Microbiology, January 2007, p. 257-258, Vol. 45, No. 1
0095-1137/07/$08.00+0     doi:10.1128/JCM.01552-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Su, H.-P., Tung, S.-K., Tseng, L.-R., Tsai, W.-C., Chung, T.-C., Chang, T. C. (2009). Identification of Legionella Species by Use of an Oligonucleotide Array. J. Clin. Microbiol. 47: 1386-1392 [Abstract] [Full Text]  


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