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Journal of Clinical Microbiology, October 2007, p. 3408-3410, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.01393-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Validation of Nasopharyngeal Sampling and Culture Techniques for Detection of Streptococcus pneumoniae in Children in Kenya{triangledown}

Osman Abdullahi,1* Eva Wanjiru,1 Robert Musyimi,1 Nina Glass,2 and J. Anthony G. Scott1,3

Kenya Medical Research Institute/Wellcome Trust Research Program, Centre for Geographic Medicine Research—Coast, Kilifi, Kenya,1 Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia,2 Nuffield Department of Clinical Medicine, Oxford University, John Radcliffe Hospital, Oxford, United Kingdom3

Received 11 July 2007/ Returned for modification 18 July 2007/ Accepted 3 August 2007

We compared nasopharyngeal swabs against nasal wash cultures for detecting colonizing pneumococci and examined the effect of frozen storage in skim milk-tryptone-glucose-glycerin on culture. Among the 55 children with positive nasal wash cultures, swab cultures were positive for 47 (85%). Of the 96 swabs positive on direct plating, 94 (98%) were positive when recultured after freezing.

* Corresponding author. Mailing address: Wellcome Trust/KEMRI Centre for Geographic Medicine Research—Coast, P.O. Box 230, Kilifi, Kenya. Phone: 254 41 525453. Fax: 254 41 522390. E-mail: oabdullahi{at}kilifi.kemri-wellcome.org

{triangledown} Published ahead of print on 15 August 2007.

Journal of Clinical Microbiology, October 2007, p. 3408-3410, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.01393-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Brugger, S. D., Hathaway, L. J., Muhlemann, K. (2009). Detection of Streptococcus pneumoniae Strain Cocolonization in the Nasopharynx. J. Clin. Microbiol. 47: 1750-1756 [Abstract] [Full Text]  


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