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Journal of Clinical Microbiology, October 2007, p. 3439-3442, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.01411-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Polymorphisms in gyrA and gyrB Genes among Mycobacterium avium subsp. paratuberculosis Type I, II, and III Isolates{triangledown}

E. Castellanos,1 A. Aranaz,1* B. Romero,1 L. de Juan,1 J. Alvarez,1 J. Bezos,1 S. Rodríguez,1 K. Stevenson,2 A. Mateos,1 and L. Domínguez1

Departamento de Sanidad Animal, Laboratorio de Vigilancia Sanitaria Veterinaria, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain,1 Moredun Research Institute, Pentlands Science Park, Penicuik EH26 OPZ, Scotland, United Kingdom2

Received 13 July 2007/ Accepted 22 July 2007

The analysis of the gyrA and gyrB genes of a panel of Mycobacterium avium subsp. paratuberculosis isolates from types I, II, and III detected type-specific single nucleotide polymorphisms. Based on these results, we developed a PCR and restriction enzyme analysis to discriminate type I and III isolates. The application of this technique would be the unique strategy to characterize these strains when there is not enough bacterial growth to perform pulsed-field gel electrophoresis and IS900 restriction fragment length polymorphism.

* Corresponding author. Mailing address: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain. Phone: 34913943721. Fax: 34913943795. E-mail: address: alaranaz{at}vet.ucm.es

{triangledown} Published ahead of print on 1 August 2007.

Journal of Clinical Microbiology, October 2007, p. 3439-3442, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.01411-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Castellanos, E., Aranaz, A., de Juan, L., Alvarez, J., Rodriguez, S., Romero, B., Bezos, J., Stevenson, K., Mateos, A., Dominguez, L. (2009). Single Nucleotide Polymorphisms in the IS900 Sequence of Mycobacterium avium subsp. paratuberculosis Are Strain Type Specific. J. Clin. Microbiol. 47: 2260-2264 [Abstract] [Full Text]  
  • Castellanos, E., Aranaz, A., Gould, K. A., Linedale, R., Stevenson, K., Alvarez, J., Dominguez, L., de Juan, L., Hinds, J., Bull, T. J. (2009). Discovery of Stable and Variable Differences in the Mycobacterium avium subsp. paratuberculosis Type I, II, and III Genomes by Pan-Genome Microarray Analysis. Appl. Environ. Microbiol. 75: 676-686 [Abstract] [Full Text]  
  • Griffiths, T. A., Rioux, K., De Buck, J. (2008). Sequence Polymorphisms in a Surface PPE Protein Distinguish Types I, II, and III of Mycobacterium avium subsp. paratuberculosis. J. Clin. Microbiol. 46: 1207-1212 [Abstract] [Full Text]  


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