This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Garg, J.
Right arrow Articles by Nath, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Garg, J.
Right arrow Articles by Nath, G.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, October 2007, p. 3443-3445, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.02367-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Evaluation of Pan-Dermatophyte Nested PCR in Diagnosis of Onychomycosis{triangledown}

Jaya Garg,1* Ragini Tilak,1 Sanjay Singh,2 Anil Kumar Gulati,1 Atul Garg,1 Pradyot Prakash,1 and Gopal Nath1

Departments of Microbiology,1 Dermatology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India2

Received 22 November 2006/ Returned for modification 12 February 2007/ Accepted 23 July 2007

In this study, nested PCR using novel primers targeting the pan-dermatophyte-specific sequence of the chitin synthase 1 gene (CHS1) was compared with KOH microscopy, culture isolation, and single-round PCR for diagnosis of 152 patients with clinically suspected onychomycosis. Results indicate that nested PCR may be considered the gold standard for the diagnosis of cases of onychomycosis for which the etiological agents are dermatophytes.

* Corresponding author. Present address: Department of Microbiology, JIPMER, Pondicherry 605006, India. Phone: 0091-9344493596. Fax: 0091-413-2272067. E-mail: jaya_bhu{at}rediffmail.com

{triangledown} Published ahead of print on 15 August 2007.

Journal of Clinical Microbiology, October 2007, p. 3443-3445, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.02367-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»