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Journal of Clinical Microbiology, November 2007, p. 3737-3742, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.01243-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Revised Culture-Based System for Identification of Malassezia Species{triangledown}

Takamasa Kaneko,1,2 Koichi Makimura,2* Michiko Abe,3 Ryoko Shiota,4 Yuka Nakamura,5 Rui Kano,6 Atsuhiko Hasegawa,6 Takashi Sugita,7 Shuichi Shibuya,8 Shinichi Watanabe,8 Hideyo Yamaguchi,2 Shigeru Abe,2 and Noboru Okamura9

Kanto Chemical Co., Inc., Marusan Bldg. 11-5, Nihonbashi Honcho, 3-Chome, Chuo-ku, Tokyo 103-0023, Japan,1 Teikyo University Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo 192-0395, Japan,2 Department of Medical Technology, Kitasato University, 1-15-1 Kitasato, Sagamihara, Kanagawa 228-8555, Japan,3 Department of Microbiology, Takinomiya General Hospital, 486 Takinomiya, Ryonan, Ayaka, Kagawa 761-2393, Japan,4 Kyoritsu Seiyaku Co. 1-12-4 Kudankita, Chiyoda-ku, Tokyo 102-0073, Japan,5 Department of Pathobiology, School of Veterinary Medicine, Nihon University, 1866, Kameino, Fujisawa Kanagawa 252-8510, Japan,6 Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan,7 Department of Dermatology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan,8 Laboratory of Microbiology and Immunology, Graduate School of Health Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan9

Received 19 June 2007/ Returned for modification 27 August 2007/ Accepted 9 September 2007

Forty-six strains of Malassezia spp. with atypical biochemical features were isolated from 366 fresh clinical isolates from human subjects and dogs. Isolates obtained in this study included 2 (4.7%) lipid-dependent M. pachydermatis isolates; 1 (2.4%) precipitate-producing and 6 (14.6%) non-polyethoxylated castor oil (Cremophor EL)-assimilating M. furfur isolates; and 37 (34.3%) M. slooffiae isolates that were esculin hydrolyzing, 17 (15.7%) that were non-tolerant of growth at 40°C, and 2 (1.9%) that assimilated polyethoxylated castor oil. Although their colony morphologies and sizes were characteristic on CHROMagar Malassezia medium (CHROM), all strains of M. furfur developed large pale pink and wrinkled colonies, and all strains of M. slooffiae developed small (<1 mm) pale pink colonies on CHROM. These atypical strains were distinguishable by the appearance of their colonies grown on CHROM. Three clinically important Malassezia species, M. globosa, M. restricta, and M. furfur, were correctly identified by their biochemical characteristics and colony morphologies. The results presented here indicate that our proposed identification system will be useful as a routine tool for the identification of clinically important Malassezia species in clinical laboratories.

* Corresponding author. Mailing address: Teikyo University Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo 192-0395, Japan. Phone and fax: 81-426-78-3256. E-mail: makimura{at}main.teikyo-u.ac.jp

{triangledown} Published ahead of print on 19 September 2007.

Journal of Clinical Microbiology, November 2007, p. 3737-3742, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.01243-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gonzalez, A., Sierra, R., Cardenas, M. E., Grajales, A., Restrepo, S., Cepero de Garcia, M. C., Celis, A. (2009). Physiological and Molecular Characterization of Atypical Isolates of Malassezia furfur. J. Clin. Microbiol. 47: 48-53 [Abstract] [Full Text]  


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