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Journal of Clinical Microbiology, November 2007, p. 3785-3787, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.01244-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Validation of a PCR-Oligochromatography Test for Detection of Trypanozoon Parasites in a Multicenter Collaborative Trial{triangledown}

F. Claes,1,2* S. Deborggraeve,2 D. Verloo,3 P. Mertens,4 J. R. Crowther,5 T. Leclipteux,4 and P. Büscher2

Faculty of Bioscience Engineering, K. U. Leuven, Department of Biosystems, Kasteelpark Arenberg 30, 3001 Leuven, Belgium,1 Department of Parasitology, Institute of Tropical Medicine, Antwerp, Belgium,2 European Food Safety Authority, Largo N. Palli 5/A, 43100 Parma, Italy,3 Coris BioConcept, Gembloux, Belgium,4 International Atomic Energy Agency, Wagramer Strasse 5, A-1400 Vienna, Austria5

Received 15 June 2007/ Returned for modification 8 August 2007/ Accepted 10 September 2007

A PCR-oligochromatography test for diagnosis of human and animal trypanosomiasis was evaluated through a multicenter ring trial with six laboratories testing a set of 21 blinded samples, resulting in qualitative data (positive or negative). Results showed an intralaboratory repeatability (accordance) of 88.7% (credible interval [CI], 84.4 to 92.5%) and an interlaboratory repeatability (concordance) of 88.1% (CI, 84.3 to 92.3%).

* Corresponding author. Mailing address: Faculty of Bioscience Engineering, K. U. Leuven, Department of Biosystems, Kasteelpark Arenberg 30, 3001 Leuven, Belgium. Phone: 32 16 32 14 33. Fax: 32 16 32 19 94. E-mail: filip.claes{at}biw.kuleuven.be

{triangledown} Published ahead of print on 19 September 2007.

Journal of Clinical Microbiology, November 2007, p. 3785-3787, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.01244-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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