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Journal of Clinical Microbiology, November 2007, p. 3804-3806, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.01654-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Evaluation of Cytomegalovirus (CMV) DNA Quantification in Dried Blood Spots: Retrospective Study of CMV Congenital Infection{triangledown}

Christelle Vauloup-Fellous,1 Aurélie Ducroux,2 Vincent Couloigner,3 Sandrine Marlin,4 Olivier Picone,5 Julie Galimand,2 Natalie Loundon,4 Françoise Denoyelle,4 Liliane Grangeot-Keros,1 and Marianne Leruez-Ville2*

Service de Microbiologie Immunologie Biologique, Assistance Publique-Hôpitaux de Paris, Hôpital Antoine Béclère, Université Paris Sud 11, 157 Avenue de la Porte de Trivaux, 92141 Clamart, France,1 Laboratoire de Virologie, Hôpital Necker-Enfants-Malades, Assistance Publique-Hôpitaux de Paris, Université Paris Descartes, EA 36-20, 149 rue de Sèvres, 75015 Paris, France,2 Service d'ORL Pédiatrique CHU Necker-Enfants-Malades, Assistance Publique-Hôpitaux de Paris, Université Paris Descartes, 149 rue de Sèvres, 75015 Paris, France,3 Service d'ORL Hôpital Trousseau, Assistance Publique-Hôpitaux de Paris, 26 Avenue du Dr. Arnold Netter, 75571 Paris, Cedex 12, France,4 Service de Gynécologie-Obstétrique, Assistance Publique- Hôpitaux de Paris, Hôpital Antoine Béclère, Université Paris Sud 11, 157 Avenue de la Porte de Trivaux, 92141 Clamart, France5

Received 20 August 2007/ Returned for modification 24 August 2007/ Accepted 13 September 2007

We compared two protocols for extracting DNA from dried blood spots for cytomegalovirus (CMV) DNA detection and quantification by real-time PCR. Both extraction methods were reliable for the retrospective diagnosis of CMV congenital infection. Quantification of CMV DNA was valuable after normalization of viral loads with albumin gene PCR amplification results.

* Corresponding author. Mailing address: Laboratoire de Virologie, Hôpital Necker-Enfants-Malades, 149 rue de Sèvres, 75015 Paris, France. Phone: 33 1 44 49 49 62. Fax: 33 1 44 49 49 60. E-mail: marianne.leruez{at}nck.aphp.fr

{triangledown} Published ahead of print on 26 September 2007.

Journal of Clinical Microbiology, November 2007, p. 3804-3806, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.01654-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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