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Journal of Clinical Microbiology, November 2007, p. 3811-3813, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.00345-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Preliminary Evaluation of a Multiplex Reverse Transcription-PCR Assay Combined with a New DNA Chip Hybridization Assay for Detecting Respiratory Syncytial Virus{triangledown}

Manuel Causse, Ángel D. García-Mayorgas, Juan B. Gutiérrez, and Manuel Casal*

Microbiology Service, Reina Sofia University Hospital, Avda. Menendez Pidal s/n, 14004, Córdoba, Spain

Received 13 February 2007/ Returned for modification 17 April 2007/ Accepted 8 September 2007

DNA chips represent a major advance in microbiology laboratories, enabling the detection of a wide range of possible pathogens using a single test. This study compared a multiplex reverse transcription-PCR combined with DNA chip hybridization (ProDect BCS RV chip; bcs Biotech) with the indirect immunofluorescence test commonly used to detect respiratory viruses. A total of 39 respiratory viruses (38 respiratory syncytial viruses [RSVs] and 1 influenza A virus) were detected in samples from 96 patients using the immunofluorescence test, while 36 viruses (34 RSV, 1 influenza A virus, and 1 influenza B virus) were detected by the DNA chip technique. Results showed a good level of agreement between the two tests for RSV detection; the incidence of other viruses was low, since samples were taken from patients with suspected bronchiolitis. DNA chips displayed high sensitivity (94.6%) and specificity (100%).

* Corresponding author. Mailing address: Microbiology Service, Reina Sofia University Hospital, Avda. Menendez Pidal s/n, 14004, Córdoba, Spain. Phone: (34) 957010432. Fax: (34) 957010419. E-mail: mi1carom{at}uco.es

{triangledown} Published ahead of print on 19 September 2007.

Journal of Clinical Microbiology, November 2007, p. 3811-3813, Vol. 45, No. 11
0095-1137/07/$08.00+0     doi:10.1128/JCM.00345-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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