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Journal of Clinical Microbiology, December 2007, p. 4048-4050, Vol. 45, No. 12
0095-1137/07/$08.00+0     doi:10.1128/JCM.00932-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Molecular Typing of a Suspected Cluster of Nocardia farcinica Infections by Use of Randomly Amplified Polymorphic DNA, Pulsed-Field Gel Electrophoresis, and Amplified Fragment Length Polymorphism Analyses{triangledown}

J. S. Kalpoe,1* K. E. Templeton,1 A. M. Horrevorts,2 H. P. Endtz,3 E. J. Kuijper,1 A. T. Bernards,1 and C. H. W. Klaassen2

Department of Medical Microbiology, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands,1 Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Weg door Jonkerbos 100, 6532 SZ Nijmegen, The Netherlands,2 Department of Microbiology and Infectious Diseases, Erasmus MC, University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands3

Received 4 May 2007/ Returned for modification 6 June 2007/ Accepted 25 September 2007

Randomly amplified polymorphic DNA (RAPD), pulsed-field gelelectrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) analyses were used to investigate a possible outbreak of Nocardia farcinica. RAPD and PFGE analyses yielded irreproducible and unsatisfactory results, respectively. AFLP analysis seem to be a promising and welcome addition for molecular analysis of Nocardia isolates.

* Corresponding author. Mailing address: Department of Medical Microbiology, E4-P, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands. Phone: (31)-71-5263931. Fax: (31)-71-5248148. E-mail: J.S.Kalpoe{at}lumc.nl

{triangledown} Published ahead of print on 3 October 2007.

Journal of Clinical Microbiology, December 2007, p. 4048-4050, Vol. 45, No. 12
0095-1137/07/$08.00+0     doi:10.1128/JCM.00932-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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