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Journal of Clinical Microbiology, February 2007, p. 317-323, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01549-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Strain Typing and Determination of Population Structure of Candida krusei by Multilocus Sequence Typing{triangledown}

Mette D. Jacobsen,1 Neil A. R. Gow,1 Martin C. J. Maiden,2 Duncan J. Shaw,1 and Frank C. Odds1*

Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom,1 The Peter Medawar Building for Pathogen Research and Department of Zoology, University of Oxford, Oxford OX1 3SY, United Kingdom2

Received 26 July 2006/ Returned for modification 11 September 2006/ Accepted 23 October 2006

A multilocus sequence typing (MLST) scheme for Candida krusei was devised, based on sequencing of six gene fragments of the species. The existence of heterozygous results for each of the six fragments sequenced confirms that C. krusei is diploid for at least part of its genome. The C. krusei MLST scheme had a discriminatory index of 0.998, making this system ideal for strain typing of C. krusei clinical isolates. MLST data for 122 independent C. krusei isolates from a range of geographical sources were analyzed by eBURST, structure, and the unweighted-pair group method using average linkages to derive a population structure comprising four subtype strain clusters. There was no evidence of geographical associations with particular subtypes. Data for pairs of isolates from seven patients showed that each patient was colonized and/or infected with strain types that were indistinguishable by MLST. The C. krusei MLST database can be accessed online at http://pubmlst.org/ckrusei/.

* Corresponding author. Mailing address: Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom. Phone and fax: 44 1224 555828. E-mail: f.odds{at}abdn.ac.uk.

{triangledown} Published ahead of print on 22 November 2006.

Journal of Clinical Microbiology, February 2007, p. 317-323, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01549-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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