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Journal of Clinical Microbiology, February 2007, p. 438-442, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01735-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Use of Enzyme-Linked Immunosorbent Assay and Dipstick Assay for Detection of Strongyloides stercoralis Infection in Humans{triangledown}

H. Rogier van Doorn,1* Rob Koelewijn,2 Henk Hofwegen,2 Henk Gilis,1 Jose C. F. M. Wetsteyn,3 Pieter J. Wismans,2 Claudine Sarfati,4 Tony Vervoort,5 and Tom van Gool1,2

Section of Parasitology, Department of Medical Microbiology,1 Division of Infectious Diseases, Tropical Medicine and AIDS, Department of Internal Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands,3 Harbor Hospital and Institute of Tropical Diseases, Rotterdam, The Netherlands,2 Laboratory of Parasitology and Department of Infectious Diseases, Saint Louis Hospital, Assistance Publique-Hôpitaux de Paris, Paris, France,4 Prince Leopold Institute of Tropical Medicine, Central Laboratory of Clinical Biology, Antwerp, Belgium5

Received 22 August 2006/ Returned for modification 10 October 2006/ Accepted 21 November 2006

A homemade enzyme-linked immunosorbent assay (ELISA) (Academic Medical Center ELISA [AMC-ELISA]) and a dipstick assay for the detection of anti-Strongyloides stercoralis antibodies in serum were developed and evaluated together with two commercially available ELISAs (IVD-ELISA [IVD Research, Inc.] and Bordier-ELISA [Bordier Affinity Products SA]) for their use in the serodiagnosis of imported strongyloidiasis. Both commercially available ELISAs have not been evaluated previously. The sensitivities of the assays were evaluated using sera from 90 patients with parasitologically proven intestinal strongyloidiasis and from 9 patients with clinical larva currens. The sensitivities of the AMC-ELISA, dipstick assay, IVD-ELISA, and Bordier-ELISA were 93, 91, 89, and 83%, respectively, for intestinal strongyloidiasis. In all tests, eight of nine sera from patients with larva currens were positive. The specificity was assessed using a large serum bank of 220 sera from patients with various parasitic, bacterial, viral, and fungal infectious diseases; sera containing autoimmune antibodies; and sera from healthy blood donors. The specificities of AMC-ELISA, dipstick assay, IVD-ELISA, and Bordier-ELISA were 95.0, 97.7, 97.2, and 97.2%, respectively. Our data suggest that all four assays are sensitive and specific tests for the diagnosis of both intestinal and cutaneous strongyloidiasis.

* Corresponding author. Mailing address: Department of Medical Microbiology, Academic Medical Center, Room L1-245, P.O. Box 22660, 1100DD Amsterdam, The Netherlands. Phone: 31 20 5666461. Fax: 31 20 6979271. E-mail: H.R.vanDoorn{at}amc.nl.

{triangledown} Published ahead of print on 6 December 2006.

Journal of Clinical Microbiology, February 2007, p. 438-442, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01735-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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