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Journal of Clinical Microbiology, February 2007, p. 572-574, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01731-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Magnetic Silica Extraction for Low-Viremia Human Immunodeficiency Virus Type 1 Genotyping{triangledown}

D. R. McClernon,* E. Ramsey, and M. StClair

Clinical Virology Department, GlaxoSmithKline, Research Triangle Park, North Carolina

Received 21 August 2006/ Returned for modification 13 October 2006/ Accepted 7 November 2006

Nucleic acid extraction and human immunodeficiency virus type 1 (HIV-1) genotyping using the NucliSens miniMAG platform and the TruGene HIV-1 genotyping kit gave HIV-1 sequence data from HIV-1-negative plasma spiked with 100 copies/ml reference HIV-1 RNA and from low-viremia clinical samples (<500 copies/ml) without the need for ultracentrifugation or nested second-round PCR.

* Corresponding author. Mailing address: Clinical Virology Department, GlaxoSmithKline, P.O. Box 13398, Research Triangle Park, NC 27709-3398. Phone: (919) 483-9425. Fax: (919) 315-0068. E-mail: daniel.r.mcclernon{at}gsk.com.

{triangledown} Published ahead of print on 22 November 2006.

Journal of Clinical Microbiology, February 2007, p. 572-574, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01731-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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