This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nantavisai, K.
Right arrow Articles by Leelayoova, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nantavisai, K.
Right arrow Articles by Leelayoova, S.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2007, p. 581-583, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01823-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Evaluation of the Sensitivities of DNA Extraction and PCR Methods for Detection of Giardia duodenalis in Stool Specimens{triangledown}

Kwannan Nantavisai,1 Mathirut Mungthin,2 Peerapan Tan-ariya,1 Ram Rangsin,3 Tawee Naaglor,2 and Saovanee Leelayoova2*

Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand,1 Department of Parasitology,2 Department of Military and Community Medicine, Phramongkutklao College of Medicine, Ratchawithi Rd., Bangkok 10400, Thailand3

Received 2 September 2006/ Returned for modification 10 October 2006/ Accepted 9 November 2006

Sensitivities of DNA extraction methods and PCR methods for Giardia duodenalis were evaluated. A combination of the most sensitive methods, i.e., FTA filter paper and a PCR protocol using RH11/RH4 and GiarF/GiarR primers, showed no significant differences compared to immunofluorescence assay in terms of their sensitivities and specificities.

* Corresponding author. Mailing address: Department of Parasitology, Phramongkutklao College of Medicine, Ratchawithi Rd., Bangkok 10400, Thailand. Phone and fax: 662 354 7761. E-mail: s_leelayoova{at}scientist.com.

{triangledown} Published ahead of print on 22 November 2006.

Journal of Clinical Microbiology, February 2007, p. 581-583, Vol. 45, No. 2
0095-1137/07/$08.00+0     doi:10.1128/JCM.01823-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Stauffer, S. H., Birkenheuer, A. J., Levy, M. G., Marr, H., Gookin, J. L. (2008). Evaluation of four DNA extraction methods for the detection of Tritrichomonas foetus in feline stool specimens by polymerase chain reaction. jvdi 20: 639-641 [Abstract] [Full Text]  
  • Ratanapo, S., Mungthin, M., Soontrapa, S., Faithed, C., Siripattanapipong, S., Rangsin, R., Naaglor, T., Piyaraj, P., Taamasri, P., Leelayoova, S. (2008). Multiple Modes of Transmission of Giardiasis in Primary Schoolchildren of a Rural Community, Thailand. Am J Trop Med Hyg 78: 611-615 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»