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Journal of Clinical Microbiology, March 2007, p. 1005-1010, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.02264-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Kodamaea ohmeri Isolates from Patients in a University Hospital: Identification, Antifungal Susceptibility, and Pulsed-Field Gel Electrophoresis Analysis{triangledown}

Jin Sol Lee,1 Jong Hee Shin,1* Mi-Na Kim,3 Sook-In Jung,2 Kyung Hwa Park,2 Duck Cho,1 Seung Jung Kee,1 Myung Geun Shin,1 Soon Pal Suh,1 and Dong Wook Ryang1

Departments of Laboratory Medicine,1 Internal Medicine, Chonnam National University Medical School, Gwangju, Korea,2 Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea3

Received 7 November 2006/ Returned for modification 6 December 2006/ Accepted 13 January 2007

Data on clinical isolates of Kodamaea (Pichia) ohmeri, an emerging fungal pathogen, are scarce. Over the past 5 years, we identified yeast isolates from nine patients with fungemia as K. ohmeri by using the API 20C system. Here, we reanalyzed these isolates first by sequencing the internal transcribed spacer 2 (ITS2) regions and then by growing the isolates on CHROMagar Candida medium and subjecting them to pulsed-field gel electrophoresis (PFGE). Based on their ITS2 sequences, six of the nine isolates were confirmed as K. ohmeri, while the others were identified as Candida haemulonii (n = 2) and Candida parapsilosis (n = 1). PFGE karyotyping of the K. ohmeri isolates revealed similar major bands, and their colonies showed a characteristic color change from pink to blue when grown on CHROMagar Candida medium for more than 48 h. For K. ohmeri, the ranges of MICs of fluconazole, voriconazole, caspofungin, and micafungin were 2 to 32 µg/ml, 0.03 to 0.5 µg/ml, 0.125 to 0.25 µg/ml, and 0.03 to 0.06 µg/ml, respectively. Restriction endonuclease analysis of genomic NotI-digested DNA (REAG-N) from isolates from different patients produced unique patterns, suggesting that the fungemia had occurred sporadically. This study determined that ITS2 sequence data, PFGE karyotypes, and CHROMagar Candida chromogenic culture medium are reliable diagnostic tools for identifying K. ohmeri while REAG-N is useful for genotyping the clinical isolates of K. ohmeri.

* Corresponding author. Mailing address: Department of Laboratory Medicine, Chonnam National University Medical School, 8 Hakdong Dongku, Gwangju 501-757, South Korea. Phone: 82 (62) 220-5342. Fax: 82 (62) 224-2518. E-mail: shinjh{at}chonnam.ac.kr.

{triangledown} Published ahead of print on 24 January 2007.

Journal of Clinical Microbiology, March 2007, p. 1005-1010, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.02264-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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