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Journal of Clinical Microbiology, March 2007, p. 1022-1023, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.02036-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Identification of Beijing Lineage Mycobacterium tuberculosis with Combined Mycobacterial Interspersed Repetitive Unit Loci 26, 31, and ETR-A{triangledown}

Pei-Ju Chin, Chen-Che Chiu, and Ruwen Jou*

Reference Laboratory of Mycobacteriology, Research and Diagnostic Center, Centers for Disease Control, Department of Health, 161 Kun-Yang St., Nan-Kang, Taipei 115, Taiwan, Republic of China

Received 3 October 2006/ Returned for modification 10 November 2006/ Accepted 11 January 2007

A rapid method for identification of Beijing lineage Mycobacterium tuberculosis is still needed in regions of tuberculosis endemicity, especially if genotyping methods are not readily accessible. After analyzing 1,557 clinical isolates, a PCR method with combined mycobacterial interspersed repetitive unit loci 26, 31, and ETR-A for differentiation of Beijing lineage isolates was established, the sensitivity and specificity of which are 94.7% and 98.5%, respectively.

* Corresponding author. Mailing address: Reference Laboratory of Mycobacteriology, Research and Diagnostic Center, Centers for Disease Control, Department of Health, 161 Kun-Yang St., Nan-Kang, Taipei 115, Taiwan, Republic of China. Phone: (886) 2-26531370. Fax: (886) 2-26531387. E-mail: rwj{at}cdc.gov.tw.

{triangledown} Published ahead of print on 24 January 2007.

Journal of Clinical Microbiology, March 2007, p. 1022-1023, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.02036-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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