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Journal of Clinical Microbiology, March 2007, p. 1045-1048, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.02350-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Identification of Burkholderia pseudomallei and Related Bacteria by Multiple-Locus Sequence Typing-Derived PCR and Real-Time PCR{triangledown}

Pierre Wattiau,1* Mieke Van Hessche,1 Heinrich Neubauer,2,{dagger} Reena Zachariah,3 Ulrich Wernery,3 and Hein Imberechts1

Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Centre, Groeselenberg 99, 1180 Brussels, Belgium,1 Institut für Mikrobiologie der Bundeswehr, Neuherbergstr. 11 D-80937 München, Germany,2 Central Veterinary Research Laboratory, PO Box 597, Dubai, United Arab Emirates3

Received 20 November 2006/ Returned for modification 3 January 2007/ Accepted 12 January 2007

Close relatedness and genomic plasticity characterizing the high-threat pathogens Burkholderia pseudomallei and Burkholderia mallei render the molecular diagnosis of these species hard to guarantee with a maximal confidence level. This article describes fast molecular assays derived from compiled sequences of housekeeping genes determined in more than 1,000 strains. The assays proved to be robust and appropriate for general detection as well as species identification purposes.

* Corresponding author. Mailing address: Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Centre, Groeselenberg 99, 1180 Brussels, Belgium. Phone: 32-23790441. Fax: 32-23790670. E-mail: Pierre.Wattiau{at}var.fgov.be.

{triangledown} Published ahead of print on 24 January 2007.

{dagger} Present address: Institute of Bacterial Infections and Zoonoses, Friedrich-Löffler-Institut, Naumburger Str. 96a, 07743 Jena, Germany.

Journal of Clinical Microbiology, March 2007, p. 1045-1048, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.02350-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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