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Journal of Clinical Microbiology, March 2007, p. 1049-1052, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.02426-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Generic Detection of Coronaviruses and Differentiation at the Prototype Strain Level by Reverse Transcription-PCR and Nonfluorescent Low-Density Microarray{triangledown} ,{dagger}

Luciano Kleber de Souza Luna,1 Volker Heiser,2 Nicolas Regamey,3 Marcus Panning,1 Jan Felix Drexler,1 Sabue Mulangu,4 Leo Poon,5 Sigrid Baumgarte,6 Bert Jan Haijema,7 Laurent Kaiser,8 and Christian Drosten1*

Clinical Virology Section, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany,1 Chipron GmbH, Berlin, Germany,2 National Heart and Lung Institute, Imperial College London, United Kingdom,3 Institut National de Recherche Biomédicale, Kinshasa, Democratic Republic of Congo,4 Department of Microbiology, University of Hong Kong, China,5 Institute of Hygiene and Environmental Health, Hamburg, Germany,6 Institute of Virology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands,7 Central Laboratory of Virology, University Hospitals of Geneva, Switzerland8

Received 3 December 2006/ Accepted 29 December 2006

A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.

* Corresponding author. Mailing address: Clinical Virology Section, Bernhard Nocht Institute for Tropical Medicine, Bernhard Nocht Str. 74, 20359 Hamburg, Germany. Phone: 49-40-42818-490. Fax: 49-40-42818-378. E-mail: drosten{at}bni-hamburg.de.

{triangledown} Published ahead of print on 17 January 2007.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, March 2007, p. 1049-1052, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.02426-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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