This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sekiguchi, J.-I.
Right arrow Articles by Kirikae, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sekiguchi, J.-I.
Right arrow Articles by Kirikae, T.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 2007, p. 979-989, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.01772-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Outbreaks of Multidrug-Resistant Pseudomonas aeruginosa in Community Hospitals in Japan{triangledown}

Jun-Ichiro Sekiguchi,1 Tsukasa Asagi,2 Tohru Miyoshi-Akiyama,1 Atsushi Kasai,2 Yukie Mizuguchi,1 Minako Araake,1 Tomoko Fujino,1 Hideko Kikuchi,2 Satoru Sasaki,2 Hajime Watari,3 Tadashi Kojima,3 Hiroshi Miki,2 Keiji Kanemitsu,4 Hiroyuki Kunishima,4 Yoshihiro Kikuchi,2 Mitsuo Kaku,4 Hiroshi Yoshikura,5 Tadatoshi Kuratsuji,1,6 and Teruo Kirikae1*

Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan,1 National Hospital Organization, Sendai Medical Center, Miyagino 2-8-8, Miyagino, Sendai 938-8520, Japan,2 Eiken Chemical Co., Ltd., 1-33-8 Hongo, Bunkyo, Tokyo 113-8408, Japan,3 Department of Infection Control and Laboratory Diagnostics, Tohoku University Graduate School of Medicine, 1-1 Seiryo, Aoba, Sendai, Miyagi 980-8574, Japan,4 Ministry of Health, Labor, and Welfare, Kasumigaseki 1-2-2, Chiyoda, Tokyo 100-8916, Japan,5 National Research Institute for Child Health and Development, Okura 2-101, Setagaya, Tokyo 157-8535, Japan6

Received 28 August 2006/ Returned for modification 23 October 2006/ Accepted 6 November 2006

We previously reported an outbreak in a neurosurgery ward of catheter-associated urinary tract infection with multidrug-resistant (MDR) Pseudomonas aeruginosa strain IMCJ2.S1, carrying the 6'-N-aminoglycoside acetyltransferase gene [aac(6')-Iae]. For further epidemiologic studies, 214 clinical isolates of MDR P. aeruginosa showing resistance to imipenem (MIC ≥ 16 µg/ml), amikacin (MIC ≥ 64 µg/ml), and ciprofloxacin (MIC ≥ 4 µg/ml) were collected from 13 hospitals in the same prefecture in Japan. We also collected 70 clinical isolates of P. aeruginosa that were sensitive to one or more of these antibiotics and compared their characteristics with those of the MDR P. aeruginosa isolates. Of the 214 MDR P. aeruginosa isolates, 212 (99%) were serotype O11. We developed a loop-mediated isothermal amplification (LAMP) assay and a slide agglutination test for detection of the aac(6')-Iae gene and the AAC(6')-Iae protein, respectively. Of the 212 MDR P. aeruginosa isolates, 212 (100%) and 207 (98%) were positive in the LAMP assay and in the agglutination test, respectively. Mutations of gyrA and parC genes resulting in amino acid substitutions were detected in 213 of the 214 MDR P. aeruginosa isolates (99%). Of the 214 MDR P. aeruginosa isolates, 212 showed pulsed-field gel electrophoresis patterns with ≥70% similarity to that of IMCJ2.S1 and 83 showed a pattern identical to that of IMCJ2.S1, indicating that clonal expansion of MDR P. aeruginosa occurred in community hospitals in this area. The methods developed in this study to detect aac(6')-Iae were rapid and effective in diagnosing infections caused by various MDR P. aeruginosa clones.

* Corresponding author. Mailing address: Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan. Phone: (81) 3 3202 7181, ext. 2838. Fax: (81) 3 3202 7364. E-mail: tkirikae{at}ri.imcj.go.jp.

{triangledown} Published ahead of print on 22 November 2006.

Journal of Clinical Microbiology, March 2007, p. 979-989, Vol. 45, No. 3
0095-1137/07/$08.00+0     doi:10.1128/JCM.01772-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kitao, T., Miyoshi-Akiyama, T., Kirikae, T. (2009). AAC(6')-Iaf, a Novel Aminoglycoside 6'-N-Acetyltransferase from Multidrug-Resistant Pseudomonas aeruginosa Clinical Isolates. Antimicrob. Agents Chemother. 53: 2327-2334 [Abstract] [Full Text]  
  • Kirikae, T., Mizuguchi, Y., Arakawa, Y. (2008). Investigation of isolation rates of Pseudomonas aeruginosa with and without multidrug resistance in medical facilities and clinical laboratories in Japan. J Antimicrob Chemother 61: 612-615 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»