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Journal of Clinical Microbiology, April 2007, p. 1234-1237, Vol. 45, No. 4
0095-1137/07/$08.00+0     doi:10.1128/JCM.02202-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Comparison of the MChip to Viral Culture, Reverse Transcription-PCR, and the QuickVue Influenza A+B Test for Rapid Diagnosis of Influenza{triangledown}

Martin Mehlmann,1 Aleta B. Bonner,2,3 John V. Williams,4,5 Daniela M. Dankbar,1 Chad L. Moore,1 Robert D. Kuchta,1 Amy B. Podsiad,4 John D. Tamerius,6 Erica D. Dawson,1,7 and Kathy L. Rowlen1,7*

Department of Chemistry and Biochemistry, UCB 215, University of Colorado at Boulder, Boulder, Colorado 80309,1 Department of Emergency Medicine, Texas A&M University HSC College of Medicine, 2401 South 31st Street, Temple, Texas 77840,2 Pediatric Emergency Medicine, Children's Hospital of Austin, 601 East 15th Street, Austin, Texas 78701,3 Department of Pediatrics, 1161 21st Avenue South, Vanderbilt University Medical Center, Nashville, Tennessee 37232,4 Department of Microbiology and Immunology, 1161 21st Avenue South, Vanderbilt University Medical Center, Nashville, Tennessee 37232,5 Quidel Corporation, 10165 McKellar Court, San Diego, California 92121,6 InDevR, LLC, 2100 Central Ave., Suite 106, Boulder, Colorado 803017

Received 26 October 2006/ Returned for modification 5 January 2007/ Accepted 3 February 2007

The performance of a diagnostic microarray (the MChip assay) for influenza was compared in a blind study to that of viral culture, reverse transcription (RT)-PCR, and the QuickVue Influenza A+B test. The patient sample data set was composed of 102 respiratory secretion specimens collected between 29 December 2005 and 2 February 2006 at Scott & White Hospital and Clinic in Temple, Texas. Samples were collected from a wide range of age groups by using direct collection, nasal/nasopharyngeal swabs, or nasopharyngeal aspiration. Viral culture and the QuickVue assay were performed at the Texas site at the time of collection. Aliquots for each sample, identified only by study numbers, were provided to the University of Colorado and Vanderbilt University teams for blinded analysis. When referenced to viral culture, the MChip exhibited a clinical sensitivity of 98% and a clinical specificity of 98%. When referenced to RT-PCR, the MChip assay exhibited a clinical sensitivity of 92% and a clinical specificity of 98%. While the MChip assay currently requires 7 to 8 h to complete the analysis, a significant advantage of the test for influenza virus-positive samples is simultaneous detection and full subtype identification for the two subtypes currently circulating in humans (A/H3N2 and A/H1N1) and avian (A/H5N1) viruses.

* Corresponding author. Mailing address: Department of Chemistry and Biochemistry, UCB 215, University of Colorado at Boulder, Boulder, CO 80309. Phone: (303) 492-5033. Fax: (303) 492-5894. E-mail: rowlen{at}colorado.edu

{triangledown} Published ahead of print on 14 February 2007.

Journal of Clinical Microbiology, April 2007, p. 1234-1237, Vol. 45, No. 4
0095-1137/07/$08.00+0     doi:10.1128/JCM.02202-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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