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Journal of Clinical Microbiology, April 2007, p. 1353-1356, Vol. 45, No. 4
0095-1137/07/$08.00+0     doi:10.1128/JCM.01761-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Milk of Cow (Bos taurus), Buffalo (Bubalus bubalis), and Goat (Capra hircus): a Better Alternative than Fetal Bovine Serum in Media for Primary Isolation, In Vitro Cultivation, and Maintenance of Leishmania donovani Promastigotes{triangledown}

M. Muniaraj,1* C. S. Lal,2 S. Kumar,2 P. K. Sinha,2 and P. Das2

Centre for Research in Medical Entomology, Indian Council of Medical Research, No. 4, Sarojini Street, Chinna Chokkikulam, Madurai 625002, India,1 Rajendra Memorial Research Institute of Medical Sciences, Indian Council of Medical Research, Agam Kuan, Patna 800 007, India2

Received 25 August 2006/ Returned for modification 22 December 2006/ Accepted 26 January 2007

Tyndalized milk of goat, cow, and buffalo was found to be a potential substitute for fetal bovine serum (FBS) in the medium for the cultivation of Leishmania donovani promastigotes. The numbers (means) of promastigotes reached 2.6 x 107, 2.3 x 107, and 2.1 x 107/ml, respectively, in the medium supplemented with 10% milk of goat, cow, and buffalo, in comparison to 1.9 x 107/ml in the control with 10% FBS. In primary isolation, the milk-supplemented medium showed that 22 out of 26 samples were positive for promastigotes (84.6%) and the cells were maintained successfully during the observed period of 6 months.

* Corresponding author. Mailing address: Centre for Research in Medical Entomology, Indian Council of Medical Research, No. 4, Sarojini Street, Chinna Chokkikulam, Madurai 625002, India. Phone: 91-452-2529881. Fax: 91-452-2530660. E-mail: mmuniaraj{at}yahoo.com

{triangledown} Published ahead of print on 7 February 2007.

Journal of Clinical Microbiology, April 2007, p. 1353-1356, Vol. 45, No. 4
0095-1137/07/$08.00+0     doi:10.1128/JCM.01761-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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