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Journal of Clinical Microbiology, April 2007, p. 1363-1365, Vol. 45, No. 4
0095-1137/07/$08.00+0     doi:10.1128/JCM.02430-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Optimization of Culture of Leptospira from Humans with Leptospirosis{triangledown}

Vanaporn Wuthiekanun,1* Wirongrong Chierakul,1,2 Direk Limmathurotsakul,1 Lee D. Smythe,3 Meegan L. Symonds,3 Michael F. Dohnt,3 Andrew T. Slack,3 Roongreung Limpaiboon,4 Yupin Suputtamongkol,5 Nicholas J. White,1,6 Nicholas P. J. Day,1,6 and Sharon J. Peacock1,6

Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand,1 Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand,2 WHO/FAO/OIE Collaborating Centre for Reference and Research on Leptospirosis, Centre for Public Health Sciences, Queensland Health Scientific Services, Brisbane, Australia,3 Medical Department, Udon Thani General Hospital, Udon Thani, Thailand,4 Department of Medicine, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand,5 Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford OX3 7LJ, United Kingdom6

Received 4 December 2006/ Returned for modification 25 January 2007/ Accepted 5 February 2007

A prospective study of 989 patients with acute febrile illness was performed in northeast Thailand to define the yield of Leptospira from four different types of blood sample. Based on a comparison of the yields from whole blood, surface plasma, deposit from spun plasma, and clotted blood samples from 80 patients with culture-proven leptospirosis, we suggest a sampling strategy in which culture is performed using whole blood and deposit from spun plasma.

* Corresponding author. Mailing address: Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand. Phone: 662 3541395. Fax: 662 3549169. E-mail: lek{at}tropmedres.ac

{triangledown} Published ahead of print on 14 February 2007.

Journal of Clinical Microbiology, April 2007, p. 1363-1365, Vol. 45, No. 4
0095-1137/07/$08.00+0     doi:10.1128/JCM.02430-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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