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Journal of Clinical Microbiology, May 2007, p. 1604-1606, Vol. 45, No. 5
0095-1137/07/$08.00+0     doi:10.1128/JCM.02447-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

False-Positive Culture Results from Patient Tissue Specimens Due to Contamination of RPMI Medium with Cryptococcus albidus{triangledown}

Lisa G. Winston,* Marguerite Roemer, Carol Goodman, and Barbara Haller

University of California, San Francisco, San Francisco General Hospital, 1001 Potrero Avenue, San Francisco, California 94110

Received 5 December 2006/ Returned for modification 5 February 2007/ Accepted 13 February 2007

Cryptococcus albidus, a rare opportunist, was isolated from biopsy specimens from three patients over 4 days. An investigation showed that the specimens had been contaminated by placement in RPMI medium. The importance of rapid communication between the microbiology laboratory, the infectious diseases/infection control division, and other involved parties in the event of unusual occurrences is highlighted.

* Corresponding author. Mailing address: San Francisco General Hospital, 1001 Potrero Avenue, Room 5H22, San Francisco, CA 94110. Phone: (415) 206-8703. Fax: (415) 206-8965. E-mail: lisa.winston{at}ucsf.edu

{triangledown} Published ahead of print on 21 February 2007.

Journal of Clinical Microbiology, May 2007, p. 1604-1606, Vol. 45, No. 5
0095-1137/07/$08.00+0     doi:10.1128/JCM.02447-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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