This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Magaret, A. S.
Right arrow Articles by Corey, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Magaret, A. S.
Right arrow Articles by Corey, L.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 2007, p. 1618-1620, Vol. 45, No. 5
0095-1137/07/$08.00+0     doi:10.1128/JCM.01405-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Optimizing PCR Positivity Criterion for Detection of Herpes Simplex Virus DNA on Skin and Mucosa{triangledown}

Amalia S. Magaret,1,4* Anna Wald,1,2,3,5 Meei-Li Huang,1 Stacy Selke,6 and Larry Corey1,3,5

Departments of Laboratory Medicine,1 Epidemiology,2 Medicine, University of Washington, Seattle, Washington,3 Programs in Biostatistics,4 Infectious Disease, Fred Hutchinson Cancer Research Center, Seattle, Washington,5 Children's Hospital and Regional Medical Center, Seattle, Washington6

Received 7 July 2006/ Returned for modification 4 September 2006/ Accepted 18 February 2007

In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cutoff, minimizes misclassification.

* Corresponding author. Mailing address: Virology Research Clinic, University of Washington, 600 Broadway, Suite 400, Seattle, WA 98122. Phone: (206) 520-4316. Fax: (206) 720-4371. E-mail: amag{at}u.washington.edu

{triangledown} Published ahead of print on 28 February 2007.

Journal of Clinical Microbiology, May 2007, p. 1618-1620, Vol. 45, No. 5
0095-1137/07/$08.00+0     doi:10.1128/JCM.01405-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schiffer, J. T., Abu-Raddad, L., Mark, K. E., Zhu, J., Selke, S., Magaret, A., Wald, A., Corey, L. (2009). Frequent Release of Low Amounts of Herpes Simplex Virus from Neurons: Results of a Mathematical Model. Sci Transl Med 1: 7ra16-7ra16 [Abstract] [Full Text]  
  • Magaret, A S, Johnston, C, Wald, A (2009). Use of the designation "shedder" in mucosal detection of herpes simplex virus DNA involving repeated sampling. Sex. Transm. Infect. 85: 270-275 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»